Tucker M D, Barton L L, Thomson B M
Sandia National Laboratories, P.O. Box 5800, Albuquerque, New Mexico 87185-1139, USA. mdtucke@sandia gov
Biotechnol Bioeng. 1998 Oct 5;60(1):88-96. doi: 10.1002/(sici)1097-0290(19981005)60:1<88::aid-bit10>3.0.co;2-4.
Intact cells of Desulfovibrio desulfuricans were immobilized in polyacrylamide gel and used to remove soluble U and Mo from water by enzymatically mediated reduction reactions in column reactors. Formate or lactate served as the electron donor and oxidized U(VI) and Mo(VI) species served as electron acceptors. Greater than 99% removal efficiencies were achieved for both metals with initial concentrations of 5 mg/L U and 10 mg/L Mo. Hydraulic residence times in the columns were between 24 and 36 h. Sulfate concentrations as high as 2000 mg/L did not inhibit reduction of U or Mo in the columns. However, nitrate inhibited uranium reduction at concentrations near 50 mg/L and inhibited molybdenum reduction at concentrations near 150 mg/L. The results indicate that enzymatic reduction of U and Mo by immobilized cells of D. desulfuricans may be a practical method for removing these contaminants from solution in continuous-flow reactors.
脱硫脱硫弧菌的完整细胞被固定在聚丙烯酰胺凝胶中，并用于在柱式反应器中通过酶介导的还原反应从水中去除可溶性铀和钼。甲酸盐或乳酸盐作为电子供体，氧化态的铀（VI）和钼（VI）物种作为电子受体。对于初始浓度为5 mg/L铀和10 mg/L钼的两种金属，去除效率均超过99%。柱中的水力停留时间在24至36小时之间。高达2000 mg/L的硫酸盐浓度不会抑制柱中铀或钼的还原。然而，硝酸盐在浓度接近50 mg/L时抑制铀的还原，在浓度接近150 mg/L时抑制钼的还原。结果表明，脱硫脱硫弧菌固定化细胞对铀和钼的酶促还原可能是一种在连续流反应器中从溶液中去除这些污染物的实用方法。
