Tucker M D, Barton L L, Thomson B M
Sandia National Laboratories, Albuquerque, NM 87185, USA.
J Ind Microbiol Biotechnol. 1998 Jan;20(1):13-9. doi: 10.1038/sj.jim.2900472.
Intact cells of Desulfovibrio desulfuricans, immobilized in polyacrylamide gel, removed Cr, Mo, Se and U from solution by enzymatic-mediated reduction reactions. Lactate or H2 served as the electron donor and the oxidized Cr(VI), Mo(VI), Se(VI) and U(VI) served as electron acceptors. Reduction of the oxidized metal species resulted in the precipitation of solid phases of the metals. Metal removal efficiencies of 86-96% were achieved for initial concentrations of 1 mM Mo, Se, and U and 0.5 mM Cr. Insoluble metal phases accumulated on both the surface and the interior of the polyacrylamide gel. In column tests conducted for U removal, effluent concentrations less than 20 micrograms L(-1) were achieved with initial concentrations of 5 mg L(-1) and 20 mg L(-1) U and residence times from 25-37 h. The enzymatic reduction of Cr, Mo, Se, and U by immobilized cells of D. desulfuricans may be a practical method for removing these metals from solution in a biological reactor.
固定在聚丙烯酰胺凝胶中的脱硫脱硫弧菌完整细胞，通过酶介导的还原反应从溶液中去除铬、钼、硒和铀。乳酸或氢气作为电子供体，氧化态的铬（VI）、钼（VI）、硒（VI）和铀（VI）作为电子受体。氧化态金属物种的还原导致金属固相沉淀。对于1 mM的钼、硒和铀以及0.5 mM的铬的初始浓度，金属去除效率达到86 - 96%。不溶性金属相积聚在聚丙烯酰胺凝胶的表面和内部。在进行的铀去除柱试验中，对于5 mg L(-1)和20 mg L(-1)的铀初始浓度以及25 - 37小时的停留时间，流出物浓度低于20微克L(-1)。脱硫脱硫弧菌固定化细胞对铬、钼、硒和铀的酶促还原可能是在生物反应器中从溶液中去除这些金属的一种实用方法。
