Koblizek M, Masojidek J, Komenda J, Kucera T, Pilloton R, Mattoo A K, Giardi M T
Department of Plant Physiology, University of South Bohemia, Ceske Budejovice, Czech Republic.
Biotechnol Bioeng. 1998 Dec 20;60(6):664-9.
We have developed a biosensor for the detection of residual triazine-, urea- and phenolic-type herbicides, using isolated photosystem II (PSII) particles from the thermophilic cyanobacterium, Synechococcus elongatus, as biosensing elements. The herbicide detection was based on the fact that, in the presence of artificial electron acceptors, the light-induced electron transfer through isolated PSII particles is accompanied by the release of oxygen, which is inhibited by the herbicide in a concentration-dependent manner. The PSII particles were immobilized between dialysis membrane and the Teflon membrane of the Clark oxygen electrode mounted in a flow cell that was illuminated. Inclusion of the antibiotic chloramphenicol in the reaction mixtures prolonged, by 50%, the lifetime of the biosensor. The use of highly active PSII particles in combination with the flow system resulted in a reusable herbicide biosensor with good stability (50% of initial activity was still remaining after 35-h use at 25 degrees C) and high sensitivity (detection limit for diuron was 5 x 10(-10) M).
我们开发了一种用于检测残留三嗪类、尿素类和酚类除草剂的生物传感器，该传感器使用从嗜热蓝藻长链聚球藻中分离出的光系统II（PSII）颗粒作为生物传感元件。除草剂检测基于这样一个事实：在人工电子受体存在的情况下，通过分离的PSII颗粒进行的光诱导电子转移伴随着氧气的释放，而除草剂会以浓度依赖的方式抑制这种释放。PSII颗粒固定在透析膜和安装在流通池中并被光照的Clark氧电极的特氟龙膜之间。在反应混合物中加入抗生素氯霉素可使生物传感器的寿命延长50%。使用高活性的PSII颗粒与流动系统相结合，得到了一种可重复使用的除草剂生物传感器，该传感器具有良好的稳定性（在25℃下使用35小时后仍保留50%的初始活性）和高灵敏度（敌草隆的检测限为5×10^(-10) M）。
