Ooi L S, Wang H, Ng T B, Ooi V E
Department of Biochemistry, Chinese University of Hong Kong, Shatin, New Territories, China.
Biochem Cell Biol. 1998;76(4):601-8.
A mannose-binding lectin was isolated from leaves of the Chinese daffodil Narcissus tazetta (family Amaryllidaceae) using a procedure that comprised extraction with aqueous buffer, ammonium sulfate precipitation, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel Blue gel and mannose-agarose, and FPLC-gel filtration on Superose 12. The lectin was adsorbed on mannose-agarose and unadsorbed on DEAE-cellulose and Affi-gel Blue gel. It was an unglycosylated homodimer with a molecular mass of 26 kDa. Analysis of the N-terminal sequence of the N. tazetta lectin revealed considerable homology to lectins from the daffodil Narcissus pseudonarcissus, the snowdrop Galanthus nivalis (family Amaryllidaceae), the tulip Tulipa, and Kidachi aloe Aloe arborescens (family Liliaceae), and the orchid lectins (family Orchidaceae). The most striking likeness exists among the Amaryllidaceae lectins. The N. tazetta lectin exhibits hemagglutinating activity toward rabbit erythrocytes.
采用以下步骤从中国水仙（石蒜科）叶片中分离出一种甘露糖结合凝集素：用水性缓冲液提取、硫酸铵沉淀、DEAE-纤维素离子交换色谱、Affi-gel Blue凝胶和甘露糖琼脂糖亲和色谱以及Superose 12快速蛋白质液相色谱凝胶过滤。该凝集素吸附于甘露糖琼脂糖，不吸附于DEAE-纤维素和Affi-gel Blue凝胶。它是一种无糖基化的同型二聚体，分子量为26 kDa。对中国水仙凝集素的N端序列分析表明，它与水仙、雪花莲（石蒜科）、郁金香、木立芦荟（百合科）以及兰花凝集素（兰科）的凝集素具有相当高的同源性。石蒜科凝集素之间的相似性最为显著。中国水仙凝集素对兔红细胞表现出血凝活性。
