Koike T, Beppu H, Kuzuya H, Maruta K, Shimpo K, Suzuki M, Titani K, Fujita K
Institute of Pharmacognosy, Fujita Health University, Mie.
J Biochem. 1995 Dec;118(6):1205-10. doi: 10.1093/oxfordjournals.jbchem.a125008.
A novel lectin was isolated from the leaf skin of "Kidachi Aloe" (Aloe arborescens Miller var. natalensis Berger) by sequential chromatographies on Sephadex G-25 gel filtration, DEAE ion exchange, and Superdex 75 gel filtration columns. The native lectin exhibited a molecular mass of about 35 kDa on both gel filtration on a Superdex 75 column and native-PAGE under nonreducing conditions. SDS-PAGE in the presence or absence of beta-mercaptoethanol revealed two distinct peptides with molecular masses of about 5.5 and 2.3 kDa, respectively, in addition to a major 9.2 kDa subunit, indicating the presence of a partially processed subunit. The N-terminal amino acid sequence of the intact subunit showed homology with that of snowdrop lectin. The native lectin showed hemagglutinating activity toward rabbit but not human and sheep erythrocytes, and specifically bound to mannose like snowdrop lectin did, indicating that the Aloe and snowdrop lectins are structurally and functionally similar proteins. In addition, the native lectin showed strong mitogenic activity toward mouse lymphocytes.
通过在Sephadex G - 25凝胶过滤柱、DEAE离子交换柱和Superdex 75凝胶过滤柱上进行连续色谱法，从“木立芦荟”（库拉索芦荟米勒变种纳塔尔芦荟伯杰）的叶皮中分离出一种新型凝集素。在Superdex 75柱上进行凝胶过滤以及在非还原条件下进行天然聚丙烯酰胺凝胶电泳时，天然凝集素的分子量约为35 kDa。在有或没有β - 巯基乙醇存在的情况下进行十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳，除了一个主要的9.2 kDa亚基外，还分别显示出两条分子量约为5.5 kDa和2.3 kDa的不同肽段，表明存在部分加工的亚基。完整亚基的N端氨基酸序列与雪花莲凝集素的N端氨基酸序列具有同源性。天然凝集素对兔红细胞有血凝活性，但对人红细胞和羊红细胞没有血凝活性，并且像雪花莲凝集素一样特异性结合甘露糖，这表明芦荟凝集素和雪花莲凝集素是结构和功能相似的蛋白质。此外，天然凝集素对小鼠淋巴细胞表现出强烈的促有丝分裂活性。
