Zeller J A, Tschoepe D, Kessler C
Department of Neurology, Christian-Albrechts-University, Kiel, Germany.
Thromb Haemost. 1999 Mar;81(3):373-7.
Platelet activation plays a central role in acute arterial stenosis as has been shown in coronary heart disease. Likewise it can be assumed to be of importance in the evolution of acute cerebral ischemia (ACI), particularly in patients with large vessel disease. Flow cytometric detection of platelet adhesion molecules as a marker of platelet activation in a group of patients with ACI and different etiologies has not been evaluated. In 72 patients with ACI and 72 controls, the exposure of activation-dependent adhesion molecules was determined using flow cytometry after immunostaining with monoclonal antibodies against CD 62, CD 63 and thrombospondin. The extent of platelet activation differed as a function of the etiology of ACI: platelets from patients with atherosclerosis of brain-supplying arteries expressed significantly more activation markers than did controls, whereas patients with cardioembolic stroke did not. By analyzing platelet adhesion molecules it is possible to describe platelet activation profiles in patients with acute cerebral ischemia. This diagnostic procedure will be useful for monitoring individualized anti-platelet therapy and may enable distinguishing different subgroups of stroke patients.
正如在冠心病中所显示的那样，血小板活化在急性动脉狭窄中起着核心作用。同样，可以认为其在急性脑缺血（ACI）的发展过程中也很重要，尤其是在患有大血管疾病的患者中。尚未评估通过流式细胞术检测血小板粘附分子作为一组不同病因的ACI患者血小板活化标志物的情况。在72例ACI患者和72例对照中，在用抗CD 62、CD 63和血小板反应蛋白的单克隆抗体进行免疫染色后，使用流式细胞术测定活化依赖性粘附分子的暴露情况。血小板活化程度因ACI的病因不同而有所差异：供应脑动脉粥样硬化患者的血小板表达的活化标志物明显多于对照组，而心源性栓塞性中风患者则不然。通过分析血小板粘附分子，可以描述急性脑缺血患者的血小板活化情况。这种诊断方法将有助于监测个体化抗血小板治疗，并可能有助于区分不同亚组的中风患者。
