Legrand C, Woimant F, Haguenau M, Caen J
INSERM U 150, Hôpital Lariboisière, Paris, France.
Nouv Rev Fr Hematol (1978). 1991;33(6):497-9.
In vitro abnormalities of platelet behaviour have been described in patients with ischemic cerebrovascular disease (ICD) suggesting that changes in platelet receptors and/or platelet a granules may occur in the circulation. We investigated the frequency of such alterations in 95 patients that were studied within a few days after acute stroke. Using specific antibodies to two intrinsic plasma membrane glycoproteins (GPs), the GPIIb-IIIa complex and GPIV, and to thrombospondin (TSP), a a-granular protein that becomes expressed on the platelet surface upon activation, we were able to distinguish two groups of patients: 16 patients presented an increased concentration of TSP on their platelets demonstrating in vivo platelet secretion. These patients could constitute a group with increased risk of thrombosis. In contrast, 20 patients presented a decreased concentration of GPIIb-IIIa and GPIV. This decrease in intrinsic plasma membrane GPs was associated with a decrease in mean platelet volume and may suggest the occurrence of platelet fragmentation in the circulation.
缺血性脑血管疾病（ICD）患者存在血小板行为的体外异常，提示循环中可能发生血小板受体和/或血小板α颗粒的变化。我们对95例急性卒中后数天内接受研究的患者进行了此类改变发生频率的调查。使用针对两种内在质膜糖蛋白（GPs），即GPIIb-IIIa复合物和GPIV，以及针对血小板反应蛋白（TSP）的特异性抗体，TSP是一种α颗粒蛋白，在激活时会在血小板表面表达，我们能够区分两组患者：16例患者血小板上TSP浓度升高，表明体内血小板发生了分泌。这些患者可能构成血栓形成风险增加的一组人群。相比之下，20例患者的GPIIb-IIIa和GPIV浓度降低。内在质膜GPs的这种降低与平均血小板体积的减小有关，可能提示循环中发生了血小板碎片化。
