Choo Y K
Division of Life Science, College of Natural Sciences, Wonkwang University, Iksan, Chunbuk, Korea.
Mol Cells. 1999 Feb 28;9(1):56-60.
It is known that gangliosides, being ubiquitous membrane components, play important roles in cell-cell recognition, differentiation and transmembrane signalling. GM3, GM1 and GD1a were detected in the rat oviduct as major gangliosides by thin-layer chromatography (TLC) analysis. The total amounts of gangliosides from the oviducts at various times after hormone injection were not much changed. In order to identify their distribution and possible changes during ovulation, frozen sections of the rat oviducts were stained with specific monoclonal antibodies (MAbs) against the ganglio-series gangliosides. GM3 and GM1 were expressed in a different manner, but GD1a and other gangliosides were not immunohistochemically detected. In the ampullar region, GM3 was expressed in all the stroma and epithelial cells, but not GM1. GM1 was also not observed in epithelial cells. Staining by anti-GM1 monoclonal antibodies revealed long and minute thread-like structures in some of the stroma cells, whereas anti-GM3 monoclonal antibodies stained the entire cytoplasm, but not the nucleus, of all the stroma and epithelial cells. Other ganglio-series gangliosides, including GD1a, were not detected to some extent in the ampullar region by immunohistochemistry. Thus, these data suggest that GM3 and GM1 are oviduct-specific gangliosides.
已知神经节苷脂作为普遍存在的膜成分，在细胞间识别、分化和跨膜信号传导中发挥重要作用。通过薄层色谱（TLC）分析，在大鼠输卵管中检测到GM3、GM1和GD1a作为主要神经节苷脂。激素注射后不同时间输卵管中神经节苷脂的总量变化不大。为了确定它们在排卵期间的分布和可能的变化，用针对神经节系列神经节苷脂的特异性单克隆抗体（MAb）对大鼠输卵管的冰冻切片进行染色。GM3和GM1以不同方式表达，但GD1a和其他神经节苷脂未通过免疫组织化学检测到。在壶腹区域，GM3在所有基质和上皮细胞中表达，但GM1不表达。在上皮细胞中也未观察到GM1。抗GM1单克隆抗体染色显示在一些基质细胞中有长而微小的丝状结构，而抗GM3单克隆抗体则对所有基质和上皮细胞的整个细胞质进行染色，但不包括细胞核。免疫组织化学在壶腹区域未检测到包括GD1a在内的其他神经节系列神经节苷脂。因此，这些数据表明GM3和GM1是输卵管特异性神经节苷脂。
