Halwa W S, Ratajczak J, Wójtowicz J G, Halwa S R
Gegenbaurs Morphol Jahrb. 1976;122(4):492-6.
The aim of the work was the analysis of distribution of karyometric measurements for semi- and ultrathin sections. The investigation was performed using spheres (diameter 16.2 mm), which were randomly cut using a microtome knife. The diameters of sections were measured with ocular with accuracy 0.1 mm. It has been shown, that the mean value of the measurements does not correspond to the real value of the spheres' diameter, and it is distinctly lower. The theoretical analysis explaining this fenomenon was performed. The obtained results allow for estimation of real diameter of spherical nuclei of the some size.
这项工作的目的是分析半薄切片和超薄切片的核测量分布情况。研究使用了球体(直径16.2毫米),用切片机刀片随机切割这些球体。用目镜测量切片的直径,精度为0.1毫米。结果表明,测量的平均值与球体直径的实际值不相符,且明显较低。对这一现象进行了理论分析。所得结果有助于估计某些大小的球形细胞核的实际直径。