Ebner K, Wotjak C T, Holsboer F, Landgraf R, Engelmann M
Max Planck Institute of Psychiatry, Kraepelinstr.2, D-80804 Munich, Germany.
Eur J Neurosci. 1999 Mar;11(3):997-1002. doi: 10.1046/j.1460-9568.1999.00508.x.
The aim of the present study was to investigate the physiological significance of the neuropeptide arginine vasopressin (AVP) released within the septum, in the behavioural response of rats to stress. In the first experiment, rats were chronically implanted with a microdialysis probe aimed at the mediolateral or ventral septum to monitor the local release of AVP in response to 10 min of forced swimming in 20 degrees C warm water. Exposure to this stressor caused a significant increase in AVP release in both the mediolateral (174 +/- 21%, P < 0.01) and ventral septum (220 +/- 33%, P < 0.01). In contrast, microdialysates collected outside the mediolateral septum or in the lateral ventricle remained at prestress levels throughout the dialysis period. Furthermore, unstressed control animals failed to show significant alterations in vasopressin release in the mediolateral septum. In a second experiment, the introduction of the V1 receptor antagonist d(CH2)5Tyr(Me)AVP into the mediolateral septum via inverse microdialysis concomitant with stressor exposure caused the rats to spend an increased time floating and a reduced time swimming compared to vehicle-treated rats. This effect was acute and also detected 24 h after antagonist administration. Taken together, these findings demonstrate a significant activation of the septal vasopressinergic system in response to swim stress. Furthermore, our data support the view that AVP released within this brain area is involved in the generation of active behavioural strategies aimed at coping with new and challenging situations.
本研究的目的是探讨中隔内释放的神经肽精氨酸加压素（AVP）在大鼠对应激的行为反应中的生理意义。在第一个实验中，将大鼠长期植入针对中外侧或腹侧中隔的微透析探针，以监测在20摄氏度温水中进行10分钟强迫游泳时AVP的局部释放。暴露于这种应激源导致中外侧（174±21%，P<0.01）和腹侧中隔（220±33%，P<0.01）的AVP释放均显著增加。相比之下，在整个透析期间，从中外侧中隔外部或侧脑室收集的微透析液保持在应激前水平。此外，未受应激的对照动物中外侧中隔的加压素释放未显示出显著变化。在第二个实验中，通过反向微透析将V1受体拮抗剂d(CH2)5Tyr(Me)AVP引入中外侧中隔，并同时暴露于应激源，与给予载体的大鼠相比，导致大鼠漂浮时间增加，游泳时间减少。这种效应是急性的，在给予拮抗剂24小时后也能检测到。综上所述，这些发现表明中隔加压素能系统在应对游泳应激时显著激活。此外，我们的数据支持这样一种观点，即该脑区内释放的AVP参与了旨在应对新的挑战性情况的积极行为策略的产生。
