Knochel W, Lange D, Hendrick D
Mol Biol Rep. 1976 Nov;3(2):143-50. doi: 10.1007/BF00423228.
Poly(A)-containing 9S RNA from chick reticulocytes was electrophoresed on formamide-polyacrylamide gels. The molecular weight was determined to be 211 000 +/- 10 000 daltons. The RNA was separated into three different fractions with respect to molecular weight. These RNAs were translated in a wheat germ cell-free system. The lower molecular weight RNA directed up to 95% alpha-chain synthesis, compared to 60% for the higher molecular weight RNA. This was accompanied by a relative increase for beta-chain synthesis with increasing molecular weight. It could also be shown by hybridization with labelled poly(U) that the average poly(A) length decreased from about 83 nucleotides for fraction I to 36 nucleotides for fraction III. Our results suggest that fractionation of avian 9 S globin mRNA by electrophoresis on formamide-polyacrylamide gels is dependent upon two parameters, namely differences in the lengths of the non-poly(A)-containing portion of the alpha and beta mRNAs and differences in the poly(A) lengths.
来自鸡网织红细胞的含聚腺苷酸（Poly(A)）的9S RNA在甲酰胺 - 聚丙烯酰胺凝胶上进行电泳。其分子量测定为211000±10000道尔顿。该RNA根据分子量被分离成三个不同的组分。这些RNA在麦胚无细胞体系中进行翻译。与较高分子量RNA指导60%的α链合成相比，较低分子量RNA指导高达95%的α链合成。这伴随着β链合成随分子量增加而相对增加。通过与标记的聚尿苷酸（poly(U)）杂交还可表明，聚腺苷酸（poly(A)）的平均长度从组分I的约83个核苷酸减少到组分III的36个核苷酸。我们的结果表明，通过在甲酰胺 - 聚丙烯酰胺凝胶上进行电泳对禽9S珠蛋白mRNA进行分级分离取决于两个参数，即α和β mRNA不含聚腺苷酸部分的长度差异以及聚腺苷酸长度差异。
