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聚腺苷酸序列对珠蛋白信使核糖核酸翻译效率的作用

Contribution of polyadenylate sequences to the translational efficiency of globin messenger RNAs.

作者信息

Parets Soler A, Gozalbo D, Zueco J, Sentandreu R

机构信息

Departament de Microbiologia, Facultat de Farmacia, Universitat de València, Spain.

出版信息

Biochem J. 1987 Sep 15;246(3):575-81. doi: 10.1042/bj2460575.

DOI:10.1042/bj2460575
PMID:3689323
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1148319/
Abstract

mRNAs from reticulocyte polysomes were fractionated by chromatography on poly(U)-Sepharose and thermal elution. The molar ratio of alpha- to beta-globin mRNA was found to be 2:1 and 1:1 respectively in short- and long-poly(A) size classes. Translational analyses indicated that the globin mRNAs containing long poly(A) tracts (with a mean length of about 70 nucleotides) directed protein synthesis with higher rates than did mRNA containing short poly(A) tracts (15-35 nucleotides). Experiments performed with sub-saturating mRNA concentrations showed that the digestion with RNAase H induced a decrease in the translational capacity of both globin mRNAs and an increase in the alpha- to beta-globin synthesis ratio. No correlation was observed between the size of the poly(A) tail in mRNA and the optimal K+ requirement for translation.

摘要

来自网织红细胞多核糖体的mRNA通过在聚（U）-琼脂糖上的色谱法和热洗脱进行分级分离。在短聚（A）和长聚（A）大小类别中，α-珠蛋白mRNA与β-珠蛋白mRNA的摩尔比分别为2:1和1:1。翻译分析表明，含有长聚（A）序列（平均长度约70个核苷酸）的珠蛋白mRNA指导蛋白质合成的速率高于含有短聚（A）序列（15 - 35个核苷酸）的mRNA。用亚饱和mRNA浓度进行的实验表明，用RNA酶H消化会导致两种珠蛋白mRNA的翻译能力下降，以及α-珠蛋白与β-珠蛋白合成比率增加。在mRNA中聚（A）尾巴的大小与翻译所需的最佳K +之间未观察到相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/def1/1148319/08de402108fb/biochemj00247-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/def1/1148319/08de402108fb/biochemj00247-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/def1/1148319/08de402108fb/biochemj00247-0025-a.jpg

相似文献

1
Contribution of polyadenylate sequences to the translational efficiency of globin messenger RNAs.聚腺苷酸序列对珠蛋白信使核糖核酸翻译效率的作用
Biochem J. 1987 Sep 15;246(3):575-81. doi: 10.1042/bj2460575.
2
Molecular modifications associated with Aging of globin messenger RNA in vitro.体外与珠蛋白信使核糖核酸老化相关的分子修饰。
Eur J Biochem. 1976 Mar 1;62(3):509-17. doi: 10.1111/j.1432-1033.1976.tb10185.x.
3
In vitro translation of globin: effect of proteins purified by affinity chromatography on polyadenylate-Sepharose.珠蛋白的体外翻译：通过聚腺苷酸-琼脂糖亲和层析纯化的蛋白质的作用。
Biochemistry. 1976 Aug 10;15(16):3529-35. doi: 10.1021/bi00661a021.
4
Molecular weights, poly(A)--content, and partial separation of chick globin mRNAs.鸡珠蛋白信使核糖核酸的分子量、聚腺苷酸含量及部分分离
Mol Biol Rep. 1976 Nov;3(2):143-50. doi: 10.1007/BF00423228.
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Molecular weight determinations on the alpha-globin and beta-globin mRNAs.对α-珠蛋白和β-珠蛋白信使核糖核酸的分子量测定。
Biochim Biophys Acta. 1976 Sep 20;447(1):104-9. doi: 10.1016/0005-2787(76)90100-3.
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Role of the 3'-poly(A) sequence in translational regulation of mRNAs in Xenopus laevis oocytes.3'-聚腺苷酸序列在非洲爪蟾卵母细胞mRNA翻译调控中的作用。
J Biol Chem. 1988 Apr 25;263(12):5764-70.
7
Shortening of the poly(A) tail at the 3'-end of mRNA requires its translation.mRNA 3' 端多聚腺苷酸尾巴的缩短需要其进行翻译。
Cell Mol Biol Res. 1993;39(1):73-80.
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The putative 15 S precursor of globin mRNA contains a poly (A) sequence.假定的珠蛋白信使核糖核酸15S前体含有一个多聚腺苷酸序列。
Biochim Biophys Acta. 1978 Jan 26;517(1):99-108. doi: 10.1016/0005-2787(78)90037-0.
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Do pyrimidine nucleotides regulate translatability of globin mRNA as purine nucleotides do?嘧啶核苷酸是否像嘌呤核苷酸那样调节珠蛋白mRNA的可翻译性？
Int J Biochem. 1988;20(10):1051-9. doi: 10.1016/0020-711x(88)90249-2.
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Organization of sequences of avian globin mRNA.鸟类珠蛋白信使核糖核酸序列的组织
Eur J Biochem. 1977 Jan;72(2):291-9. doi: 10.1111/j.1432-1033.1977.tb11252.x.

本文引用的文献

1
Messenger RNA stability in Dictyostelium discoideum: does poly(A) have a regulatory role?盘基网柄菌中信使核糖核酸的稳定性：聚腺苷酸是否具有调节作用？
J Mol Biol. 1980 Aug 5;141(2):99-118. doi: 10.1016/0022-2836(80)90379-4.
2
The role of mRNA competition in regulating translation. IV. Kinetic model.mRNA竞争在调节翻译中的作用。IV. 动力学模型。
J Biol Chem. 1981 Nov 25;256(22):11762-73.
3
Requirement of different ionic concentrations for optimal translation of alpha and beta globin mRNA in Krebs II Ascites cell-free system.在克雷布斯II腹水无细胞体系中，α和β珠蛋白mRNA最佳翻译所需的不同离子浓度
Biochem Biophys Res Commun. 1981 Apr 30;99(4):1427-32. doi: 10.1016/0006-291x(81)90778-6.
4
Sequence-specific adenylations and deadenylations accompany changes in the translation of maternal messenger RNA after fertilization of Spisula oocytes.在鸟蛤卵母细胞受精后，序列特异性腺苷化和去腺苷酸化伴随着母源信使核糖核酸翻译的变化。
J Mol Biol. 1983 May 25;166(3):309-27. doi: 10.1016/s0022-2836(83)80087-4.
5
Role of mRNA competition in regulating translation: further characterization of mRNA discriminatory initiation factors.mRNA竞争在调节翻译中的作用：mRNA识别起始因子的进一步表征
Proc Natl Acad Sci U S A. 1983 Feb;80(3):663-7. doi: 10.1073/pnas.80.3.663.
6
The rate of initiation of alpha- and beta-globin mRNA translation is modulated by 50 kDa, 28-kDa and 24-kDa polypeptide-containing fractions.含有50 kDa、28 kDa和24 kDa多肽的组分可调节α-和β-珠蛋白mRNA的翻译起始速率。
Biochem Biophys Res Commun. 1984 May 16;120(3):804-11. doi: 10.1016/s0006-291x(84)80178-3.
7
Preferential stimulation of rabbit alpha globin mRNA translation by a cap-binding protein complex.帽结合蛋白复合物对兔α珠蛋白mRNA翻译的优先刺激作用。
Biochim Biophys Acta. 1984 Nov 22;783(2):122-9. doi: 10.1016/0167-4781(84)90003-4.
8
Translational control by messenger RNA competition for eukaryotic initiation factor 2.信使核糖核酸对真核起始因子2的竞争所介导的翻译调控
J Biol Chem. 1982 Jan 25;257(2):946-52.
9
The role of mRNA competition in regulating translation. III. Comparison of in vitro and in vivo results.信使核糖核酸竞争在调节翻译中的作用。III. 体外和体内结果的比较。
J Biol Chem. 1981 Nov 25;256(22):11755-61.
10
Translational activity and functional stability of human fibroblast beta 1 and beta 2 interferon mRNAs lacking 3'-terminal RNA sequences.缺乏3'-末端RNA序列的人成纤维细胞β1和β2干扰素mRNA的翻译活性和功能稳定性。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1741-5. doi: 10.1073/pnas.78.3.1741.