Peggy L, Jacques P
Paroi Arterielle. 1976 Oct;3(4):145-50.
The enzymes involved in glycosaminoglycans biosynthesis are characterized in homogenates of aortic media-intima. Incorporation of hexosamine from uridine nucleotide precursor was done into the saccharide chains of glycosaminoglycans. Labelling of aortic glycosaminoglycans by 35S-precursors is performed in our fractions; the results obtained demonstrate the uptake of 35S-sulfate from its corresponding active nucleotide sulfate ; highest sulfation is obtained for the post-microsomal supernatant. Following incorporation of 35S-sulfate or 14C-radio-activity into the different glycosaminoglycans, the largest percent uptake is obtained for heparan sulfate and the lowest for chondroitin sulfate.
参与糖胺聚糖生物合成的酶在主动脉中膜 - 内膜匀浆中得到表征。将来自尿苷核苷酸前体的己糖胺掺入糖胺聚糖的糖链中。在我们的组分中用35S前体对主动脉糖胺聚糖进行标记；所得结果表明从其相应的活性核苷酸硫酸盐中摄取了35S - 硫酸盐；微粒体后上清液的硫酸化程度最高。在将35S - 硫酸盐或14C放射性掺入不同的糖胺聚糖后，硫酸乙酰肝素的摄取百分比最高，硫酸软骨素的摄取百分比最低。
