Levy P, Picard J, Bruel A
Paroi Arterielle. 1981;7(3):113-9.
We investigated "in vitro" incorporation of labelled (14C) - or (35S) - precursors into microsomal heparan sulfate of pig aortic media-intima. Heparan sulfate, predominantly located on the external surface of smooth muscle cells, may conceivably provide a mean for the selective binding of plasma components to the arterial wall; functional implications of such binding, have been proposed in atherogenesis and thrombogenesis processes. We demonstrated the great metabolic activity of heparan sulfate chains. By evaluation of radioactivity incorporated into monosaccharides residues, we showed that sugar was quantitatively transferred from UDPGlcNAc 14C into endogenous glycosaminoglycans. The relationship between the N- and O-sulfation processes into heparan sulfate chains was studied after different time of sulfation by labelled sulfate nucleotide: PAP(35S). Our results outlined that preferential N-sulfation is obtained with short time exposures to labelled precursor, the O-sulfation occurring on previously N-sulfated heparan sulfate.
我们研究了标记的（14C）-或（35S）-前体“体外”掺入猪主动脉中膜-内膜微粒体硫酸乙酰肝素的情况。硫酸乙酰肝素主要位于平滑肌细胞的外表面，可以想象它可能为血浆成分与动脉壁的选择性结合提供一种方式；这种结合在动脉粥样硬化和血栓形成过程中的功能意义已被提出。我们证明了硫酸乙酰肝素链具有很高的代谢活性。通过评估掺入单糖残基中的放射性，我们表明糖从UDPGlcNAc 14C定量转移到内源性糖胺聚糖中。在用标记的硫酸核苷酸：PAP（35S）进行不同时间的硫酸化后，研究了硫酸乙酰肝素链中N-和O-硫酸化过程之间的关系。我们的结果表明，短时间暴露于标记前体可获得优先的N-硫酸化，O-硫酸化发生在先前已进行N-硫酸化的硫酸乙酰肝素上。
