Ghetie V, Medesan C, Sjöquist J
Scand J Immunol. 1976;5(10):1199-207. doi: 10.1111/j.1365-3083.1976.tb00263.x.
A new rosette technique for identification of Fc-receptor-bearing cells is based on the ability of sheep erythrocytes coated with protein A of Staphylococcus aureus (ES) to form rosettes with cells treated with monomeric IgG or aggregated IgG. The IgG is attached to lymphocytes through its Fc region and binds to a trypsin-resistant but pronase-sensitive receptor (considered an Fc receptor). The ES rosette technique facilitates studies of the interaction of IgG with Fc receptor sites; the binding of any IgG preparation reacting with protein A of Staphylococcus aureus (SpA) can be studied by the technique. Inhibition of rosette formation by SpA was used for quantitation of IgG fixed to the cell surface (that is, the number of Fc receptors/cell). The sensitivity of the method permits quantitation of less than 10(5) IgG molecules bound to the Fc receptors. The relative affinity constant between Fc receptors and IgG ligands was estimated by plotting the percentage of ES rosettes as a function of IgG concentration and calculating the reciprocal of the IgG concentration giving half the maximal number of ES rosettes.
一种用于鉴定携带Fc受体细胞的新玫瑰花结技术，是基于用金黄色葡萄球菌蛋白A包被的绵羊红细胞（ES）与经单体IgG或聚合IgG处理的细胞形成玫瑰花结的能力。IgG通过其Fc区域附着于淋巴细胞，并与一种抗胰蛋白酶但对链霉蛋白酶敏感的受体（被认为是Fc受体）结合。ES玫瑰花结技术有助于研究IgG与Fc受体位点的相互作用；该技术可用于研究任何与金黄色葡萄球菌蛋白A（SpA）反应的IgG制剂的结合情况。利用SpA对玫瑰花结形成的抑制作用来定量固定在细胞表面的IgG（即每个细胞的Fc受体数量）。该方法的灵敏度允许定量少于10⁵个与Fc受体结合的IgG分子。通过绘制ES玫瑰花结的百分比作为IgG浓度的函数，并计算产生半数最大ES玫瑰花结数量的IgG浓度的倒数，来估计Fc受体与IgG配体之间的相对亲和常数。
