未成熟大鼠分离的睾丸间质细胞中促黄体生成素刺激睾酮生成的激素调节：垂体切除术、促卵泡激素和雌二醇-17β的作用。

Hormonal regulation of LH stimulation of testosterone production in isolated Leydig cells of immature rats: the effect of hypophysectomy, FSH, and estradiol-17beta.

作者信息

van Beurden W M, Roodnat B, de Jong F H, Mulder E, van der Molen H J

出版信息

Steroids. 1976 Dec;28(6):847-66. doi: 10.1016/0039-128x(76)90035-0.

Abstract

Testosterone production in isolated Leydig cells from testes of immature and adult rats was stimulated by addition of LH in a dose dependent way. Hypophysectomy of adult rats had no influence on LH-stimulated testosterone production in isolated Leydig cells after 5 days. In contrast hypophysectomy of immature rats resulted after 5 days in an almost complete loss of LH sensitivity of isolated Leydig cells. Daily adminitration of FSH during 5 days starting immediately after hypophysectomy maintained LH responsiveness of isolated Leydig cells of immature rats. Also FSH administration starting on day 5 after hypophysectomy resulted in a restoration of LH responsiveness. Estradiol benzoate, injected simultaneously with FSH, abolished the FSH-induced LH responsiveness.

摘要

在未成熟和成年大鼠睾丸分离的睾丸间质细胞中，促黄体生成素（LH）以剂量依赖性方式刺激睾酮生成。成年大鼠垂体切除术后5天，对分离的睾丸间质细胞中LH刺激的睾酮生成没有影响。相比之下，未成熟大鼠垂体切除术后5天，分离的睾丸间质细胞对LH的敏感性几乎完全丧失。垂体切除术后立即开始连续5天每日给予促卵泡生成素（FSH），可维持未成熟大鼠分离的睾丸间质细胞对LH的反应性。垂体切除术后第5天开始给予FSH也可使LH反应性恢复。与FSH同时注射的苯甲酸雌二醇可消除FSH诱导的LH反应性。