与合成抗原共价连接的肽聚糖激发抗噬菌体抗体的佐剂效应。
Adjuvant effect of a peptidoglycan attached covalently to a synthetic antigen provoking anti-phage antibodies.
作者信息
Langbeheim H, Arnon R, Sela M
出版信息
Immunology. 1978 Oct;35(4):573-9.
The synthetic antigen denoted P2-A--L, comprising the fragment P2 of the coat protein of MS-2 coliphage attached to multichain poly-DL-alanine, served for the immunization of guinea-pigs. Immunization was carried out either in phosphate buffered saline (PBS) or in Freund's incomplete adjuvant (FIA) in the presence or absence of a small molecular weight peptidoglycan prepared from Bacillus megaterium, which was checked for its adjuvant effect. The various antisera were assessed by their capacity to neutralize MS-2 bacteriophage viability. When injected in PBS or FIA, P2-A--L did not elicit any measurable anti-phage activity. Addition of the peptidoglycan by simple mixing did not bring about a significant increase in antibody production. However, when the peptidoglycan was chemically linked to the P2-A--L conjugate, it had a marked adjuvant effect when the material was administered in FIA, almost identical to the extent of the effect of Freund's complete adjuvant.
合成抗原P2-A--L由附着在多链聚-DL-丙氨酸上的MS-2噬菌体外壳蛋白的P2片段组成，用于免疫豚鼠。免疫在磷酸盐缓冲盐水（PBS）中或弗氏不完全佐剂（FIA）中进行，存在或不存在由巨大芽孢杆菌制备的小分子量肽聚糖，并检测其佐剂效果。通过中和MS-2噬菌体活力的能力评估各种抗血清。当在PBS或FIA中注射时，P2-A--L不会引发任何可测量的抗噬菌体活性。通过简单混合添加肽聚糖不会导致抗体产生显著增加。然而，当肽聚糖化学连接到P2-A--L偶联物上时，当该物质在FIA中给药时具有明显的佐剂作用，几乎与弗氏完全佐剂的作用程度相同。
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