豚鼠对金黄色葡萄球菌肽聚糖的迟发型超敏反应。

Delayed type hypersensitivity to Staphylococcus aureus peptidoglycan in guinea pigs.

作者信息

Dziarski R

出版信息

Zentralbl Bakteriol Orig A. 1978 Apr;240(2):165-70.

Abstract

Skin hypersensitivity to Staphylococcus aureus peptidoglycan in guinea pigs was studied. For distinct skin reaction injection of 50 microgram of the antigen was required. Nonsensitized guinea pigs and animals sensitized with emulsion of 1 mg or 100 microgram of peptidoglycan with complete or incomplete FREUND's adjuvant exhibited distinct immediate skin hypersensitivity. Nonsensitized animals did not show delayed type hypersensitivity (DTH). For development of DTH sensitization with 100 microgram of the antigen with incomplete FREUND's adjuvant was sufficient. The application of complete FREUND's adjuvant resulted in intensification of DTH; however, also nonspecific sensitization to staphylococcal peptidoglycan with Mycobacterium butyricum cells, present in complete adjuvant, occurred.

摘要

对豚鼠皮肤对金黄色葡萄球菌肽聚糖的超敏反应进行了研究。为了产生明显的皮肤反应，需要注射50微克抗原。未致敏的豚鼠以及用1毫克或100微克肽聚糖与完全或不完全弗氏佐剂乳化液致敏的动物表现出明显的即刻皮肤超敏反应。未致敏的动物未表现出迟发型超敏反应（DTH）。为了产生DTH，用100微克抗原与不完全弗氏佐剂致敏就足够了。使用完全弗氏佐剂会导致DTH增强；然而，完全佐剂中存在的丁酸分枝杆菌细胞也会引起对葡萄球菌肽聚糖的非特异性致敏。