Muylle L, Vanderplanken M, Goossens W, Stewart M, Payrat J M
Blood Transfusion Center Antwerp, The Belgian Red Cross, Edegem, Belgium.
Transfus Sci. 1994 Jun;15(2):163-9. doi: 10.1016/0955-3886(94)90091-4.
Units of whole blood collected into butyryl-n-trihexyl-citrate (BTHC) and diethylhexyl phtalate (DEHP) plasticized polyvinylchloride (PVC) blood storage containers and processed by means of an 'Optipress', which allows automated removal of the buffy coat, were compared. Units collected into standard PVC containers processed by the traditional method (no buffy coat removal) were used as a control group. The red cell concentrates were suspended in saline-adenine-glucose-mannitol (SAGM) and stored 42 days at 2-6 degrees C. Comparison of the buffy coat depleted red cell concentrates showed that red cell energy and oxygen delivery capacity, as evidenced by ATP and 2,3-DPG values, were slightly better preserved in the BTHC plasticized container, compared to the DEHP container. The red cell membrane, however, was slightly less well preserved, (the hemolysis at day 42 with BTHC ww 0.39%; with DEHP, 0.20%) in this container. The higher ATP levels might lead to a better in vivo recovery of stored red cells. In vivo studies comparing both plastic containers, therefore are indicated in order to determine if these differences have practical significance. A longer holding time of the whole blood at room temperature before processing reduced the hemolysis (42 days stored RCC as 0.26%). Slightly more fibrinopeptide A (FPA) generation and marginally lower pH and 2,3-DPG values were observed in this situation. This finding suggests an effect of higher plasticizer levels on the red cell membrane.
将采集到含丁酰 - n - 三己基柠檬酸酯（BTHC）和邻苯二甲酸二异辛酯（DEHP）增塑聚氯乙烯（PVC）储血容器中的全血单位，通过“Optipress”进行处理（该设备可自动去除白膜层），并与采用传统方法（不去除白膜层）处理的标准PVC容器中采集的全血单位进行比较。红细胞浓缩液悬浮于生理盐水 - 腺嘌呤 - 葡萄糖 - 甘露醇（SAGM）中，在2 - 6℃下储存42天。对去除白膜层的红细胞浓缩液的比较表明，从ATP和2,3 - 二磷酸甘油酸（2,3 - DPG）值可看出，与DEHP容器相比，BTHC增塑容器中的红细胞能量和氧输送能力保存得稍好一些。然而，该容器中红细胞膜的保存稍差（42天时BTHC组的溶血率为0.39%；DEHP组为0.20%）。较高的ATP水平可能导致储存红细胞在体内的恢复情况更好。因此，需要进行体内研究以比较这两种塑料容器，来确定这些差异是否具有实际意义。全血在处理前在室温下放置较长时间可降低溶血率（储存42天的红细胞浓缩液溶血率为0.26%）。在这种情况下，观察到纤维蛋白肽A（FPA）生成略有增加，pH值和2,3 - DPG值略有降低。这一发现表明较高的增塑剂水平对红细胞膜有影响。
