Trinh M
Department of Clinical Pathology, Medical College of Georgia, Augusta 30912.
Clin Lab Sci. 1991 Jan-Feb;4(1):45-7.
White blood cell (WBC) counts were compared in the presence of "backlighting" using the Coulter S-Plus Jr and a manual system. Platelet aggregates and large platelets in specimens anticoagulated with ethylenediamine tetraacetic acid (EDTA) cause interference and prompt the WBC count on the Coulter S-Plus Jr to backlight. This means that the background behind the count on the data terminal display lights up, alerting the operator to a questionable result. A total of 29 automated backlighted WBC counts of blood samples collected in sodium and potassium salts of EDTA tubes were compared with the results by hemocytometer method. Values for WBC count by both methods showed good agreement. Smear examination detected platelet clumps and large platelets in 66% of the Na 2EDTA tubes. Only low WBC counts prompted backlighting in K 3EDTA. tubes. Liquid K 3EDTA is a preferred anticoagulant for whole blood analysis because of its rapid solubility, eliminating clumping of platelets and thus backlighting.
使用库尔特S-Plus Jr血细胞分析仪和手工系统，在存在“背光”的情况下对白细胞（WBC）计数进行了比较。用乙二胺四乙酸（EDTA）抗凝的标本中的血小板聚集物和大血小板会产生干扰，并促使库尔特S-Plus Jr血细胞分析仪上的白细胞计数出现背光现象。这意味着数据终端显示屏上计数背后的背景会亮起，提醒操作人员结果可能有问题。将用EDTA钠盐和钾盐管采集的血液样本的29次自动背光白细胞计数结果与血细胞计数板法的结果进行了比较。两种方法的白细胞计数结果显示出良好的一致性。涂片检查在66%的Na₂EDTA管中检测到血小板团块和大血小板。只有低白细胞计数会促使K₃EDTA管出现背光现象。液态K₃EDTA是全血分析的首选抗凝剂，因为它溶解迅速，可消除血小板聚集，从而避免背光现象。
