大鼠脂肪组织匀浆中甘油磷脂合成的磷酸甘油、磷酸二羟丙酮和单酰甘油途径。
The glycerol phosphate, dihydroxyacetone phosphate and monoacylglycerol pathways of glycerolipid synthesis in rat adipose-tissue homogenates.
作者信息
Dodds P F, Gurr M I, Brindley D N
出版信息
Biochem J. 1976 Dec 15;160(3):693-700. doi: 10.1042/bj1600693.
Abstract
- Fat-free homogenates from the epididymal fat-pads of rats were used to measure the rate of palmitate esterification with different substrates. The effectiveness of the acyl acceptors decreased in the order glycerol phosphate, dihydroxyacetone phosphate, 2-octadecenyl-glycerol and 2-hexadecylglycerol. 2. Glycerol phosphate and dihydroxyacetone phosphate inhibited their rates of esterification in a mutually competitive manner. 3. The esterification of glycerol phosphate was also inhibited in a partially competitive manner by 2-octadecenylglycerol and to a lesser extent by 2-hexadecylglycerol. However, glycerol phosphate did not inhibit the esterification of 2-octadecenylglycerol. 4. The esterification of dihydroxyacetone phosphate and 2-hexadecylglycerol was more sensitive to inhibition by clofenapate than was that of glycerol phosphate. Norfenfluramine was more effective in inhibiting the esterification of 2-hexadecylglycerol than that of glycerol phosphate or dihydroxyacetone phosphate. 5 It is concluded that rat adipose tissue can synthesize glycerolipids by three independent routes.
摘要
- 从大鼠附睾脂肪垫中提取的无脂匀浆用于测量不同底物存在时棕榈酸酯化的速率。酰基受体的有效性按磷酸甘油、磷酸二羟丙酮、2-十八碳烯基甘油和2-十六烷基甘油的顺序降低。2. 磷酸甘油和磷酸二羟丙酮以相互竞争的方式抑制它们的酯化速率。3. 磷酸甘油的酯化也受到2-十八碳烯基甘油的部分竞争性抑制，并在较小程度上受到2-十六烷基甘油的抑制。然而，磷酸甘油并不抑制2-十八碳烯基甘油的酯化。4. 与磷酸甘油相比，磷酸二羟丙酮和2-十六烷基甘油的酯化对氯苯那酯的抑制更敏感。氟苯丙胺在抑制2-十六烷基甘油的酯化方面比抑制磷酸甘油或磷酸二羟丙酮的酯化更有效。5. 得出的结论是，大鼠脂肪组织可以通过三条独立途径合成甘油脂质。
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