Werkmeister J A, White J F, Edwards G A, Ramshaw J A
CSIRO Division of Biomolecular Engineering, Parkville, Victoria, Australia.
J Long Term Eff Med Implants. 1995;5(1):1-10.
The search for the ideal vascular prosthesis for below the knee replacement or coronary surgery continues. Long-term explant analysis of a biosynthetic composite vessel, the Omniflow Vascular Prosthesis I, has previously shown sound structural durability. An important determinant in defining durability has been the evaluation of the degree of persistence of the original biological components and the augmentation by new tissue. The development of a panel of specific monoclonal antibodies to collagens, that allow detection of new host collagen among the original collagens of the prosthesis has been a key factor. In the present study, these antibodies have been used to investigate the degree and rate of new collagen tissue infiltration in an improved version of the Omniflow prosthesis. The data show that new collagen types III and VI can be detected as early as 3 months in the adventitial tissue, and that complete tissue augmentation throughout the entire vessel wall is apparent by 6 months after implant. The novel explant analyses serve as a good predictive indication to the in vivo performance of the device and would be useful in rapid monitoring of further modifications to this vessel or to other collagen-based prostheses.
对用于膝关节以下置换或冠状动脉手术的理想血管假体的研究仍在继续。先前对一种生物合成复合血管——全流血管假体I进行的长期植入物分析表明其具有良好的结构耐久性。定义耐久性的一个重要决定因素是评估原始生物成分的持续程度以及新组织的增加情况。开发一组针对胶原蛋白的特异性单克隆抗体，能够在假体的原始胶原蛋白中检测出新的宿主胶原蛋白，这是一个关键因素。在本研究中，这些抗体被用于研究全流假体改进版中新胶原组织浸润的程度和速率。数据表明，早在植入后3个月，在外膜组织中就能检测到新的III型和VI型胶原蛋白，并且在植入后6个月，整个血管壁的完全组织增生就很明显了。这种新颖的植入物分析为该装置的体内性能提供了良好的预测指标，并且有助于快速监测该血管或其他基于胶原蛋白的假体的进一步改进。
