Gutensohn K, Sputtek A, Voss A, Stahl R A, Kuehnl P
Department of Transfusion Medicine/Transplantation Immunology, University Hospital Eppendorf, University of Hamburg, Germany.
Transfus Sci. 1996 Dec;17(4):489-92.
In particular, activated platelets are thought to be involved in the pathophysiology of thrombotic occlusions of vessels. In this study, we evaluated activation-dependent changes in platelet antigens during extracorporeal haemodialysis treatment. Flow cytometry was used in combination with monoclonal antibodies that bind to platelet glycoproteins CD62p (GMP-140) and CD63 (GP53). Maximum peaks of mean channel fluorescence intensity (MCFI) were reached after 60 min in 20/26 procedures in CD62p (P < 0.005) and in 15/25 treatments in CD63 (p < 0.002), respectively. An initial peak of CD62p and CD63 fluorescence expression could be detected in 21/25 and 23/25 treatments, respectively (CD62p within 15, CD63 within 30 min), indicating the early onset of activation. The structural antigen CD41a MCFI slightly decreased over time in all treatments, while CD42b expression did not change. From these results we conclude that haemodialysis contributes to platelet activation and secondary hypercoagulability. Analysis of platelet glycoproteins by flow cytometry may provide clinical information on patients at a higher risk for thrombosis and may help in further improvement of haemodialysis equipment.
特别地，活化血小板被认为参与血管血栓性闭塞的病理生理学过程。在本研究中，我们评估了体外血液透析治疗期间血小板抗原的活化依赖性变化。流式细胞术与结合血小板糖蛋白CD62p（GMP-140）和CD63（GP53）的单克隆抗体联合使用。在20/26次操作中，CD62p在60分钟后达到平均通道荧光强度（MCFI）的最大峰值（P < 0.005），在15/25次治疗中，CD63在60分钟后达到最大峰值（p < 0.002）。在21/25次和23/25次治疗中，分别可以检测到CD62p和CD63荧光表达的初始峰值（CD62p在15分钟内，CD63在30分钟内），表明活化早期开始。在所有治疗中，结构抗原CD41a的MCFI随时间略有下降，而CD42b表达没有变化。从这些结果我们得出结论，血液透析导致血小板活化和继发性高凝状态。通过流式细胞术分析血小板糖蛋白可能为血栓形成风险较高的患者提供临床信息，并可能有助于进一步改进血液透析设备。
