Zingsem J, Weisbach V, Zimmermann R, Greil J, Beck J D, Neidhardt B, Eckstein R
Department of Transfusion Medicine and Haemostaseology, Friedrich-Alexander Universität, Erlangen-Nümberg, Germany.
Transfus Sci. 1996 Dec;17(4):607-10.
We performed 28 separations in 11 children weighing from 14 to 72 kg (median: 27 kg) using either the Cobe Spectra or the Fresenius AS 104 cell separator. For children under 20 kg, human albumin solution was used for the last 200 mL of the priming procedure instead of NaCl. Blood flow was reduced to 30-50 mL min-1 depending on the children's size and weight to prevent citrate reactions. Within 149-337 min we processed three times the patients' total blood volume and collected 309.7 x 10(6) (102.9-1140.0) mononuclear cells (MNC) and 2.01 x 10(6) (1.90-6.50) CD34+ cells per kg body weight. These results were comparable to the results we previously obtained in adults. We conclude from these initial results that peripherae blood stem cells (PBSC) separations, with both devices, can be adapted successfully to the needs of paediatric patients.
我们使用Cobe Spectra或Fresenius AS 104细胞分离器对11名体重在14至72千克（中位数：27千克）的儿童进行了28次分离。对于体重不足20千克的儿童，在预充程序的最后200毫升中使用人白蛋白溶液而非氯化钠。根据儿童的体型和体重，将血流量降至30 - 50毫升/分钟，以防止枸橼酸盐反应。在149 - 337分钟内，我们处理了患者三倍的全血量，每千克体重收集到309.7×10⁶（102.9 - 1140.0）个单核细胞（MNC）和2.01×10⁶（1.90 - 6.50）个CD34⁺细胞。这些结果与我们之前在成人中获得的结果相当。从这些初步结果中我们得出结论，使用这两种设备进行外周血干细胞（PBSC）分离都可以成功地适应儿科患者的需求。
