Zingsem J, Weisbach V, Zimmermann R, Glaser A, Greil J, Beck J D, Neidhardt B, Eckstein R
Dept. of Transfusion Medicine and Haemostaseology, Friedrich-Alexander Universität Erlangen-Nilrnberg, Germany.
Bone Marrow Transplant. 1996 Sep;18 Suppl 1:S15-6.
We performed 28 separations in 10 children from 14 to 44 kg (median: 24 kg) using either the Cobe Spectra or the Fresenius AS 104 cell separator. For children below 20 kg, human albumin solution was used for the last 200 ml of the priming procedure instead of NaCl. Blood flow was reduced to 30 - 50 ml/min depending on the childrens' size and weight to prevent citrate reactions. Within 149 - 337 minutes we processed 3 x the patients' total blood volume and collected 326.9 x 10(6) (76.5-1, 140.0) MNC and 2.03 x 10(6) (0.14-10.12) CD34+ cells per kg body weight. These results were comparable to the results we previously obtained in adults. We conclude from these initial results that PBSC separations with both devices can be adapted successfully to the needs for paediatric patients.
我们使用Cobe Spectra或费森尤斯AS 104血细胞分离机，对10名体重在14至44千克（中位数：24千克）的儿童进行了28次分离。对于体重低于20千克的儿童，在预充程序的最后200毫升中使用人白蛋白溶液而非氯化钠。根据儿童的体型和体重，将血流速度降至30 - 50毫升/分钟，以防止枸橼酸盐反应。在149 - 337分钟内，我们处理了患者3倍的全血容量，每千克体重收集到326.9×10⁶（76.5 - 1,140.0）个单核细胞和2.03×10⁶（0.14 - 10.12）个CD34⁺细胞。这些结果与我们之前在成人中获得的结果相当。从这些初步结果中我们得出结论，使用这两种设备进行外周血干细胞分离都能够成功地适应儿科患者的需求。
