Musser D A, Fiel R J
Cancer Biochem Biophys. 1976;1(6):289-94.
Differences noted in enzyme II directed RNA synthesis under varying salt conditions in nuclei isolated from uninfected and Friend virus (FV)-infected spleen cells, have been attributed to chromosomal modifications (Babcock and Rich 1973). This investigation was undertaken to determine if compositional changes occur in the chromatin of FV-infected spleens, which correlate with an altered rate of synthesis by enzyme II. A quantitative study of the chromatin constituents at various times after infection indicated that they vary in the same temporal manner as the rate of RNA synthesis in isolated nuclei. Relative to DNA, RNA, histone, and nonhistone protein reached a maximum at 14 days postinfection. This was followed by a gradual decrease during the remainder of the infection. Chromatin endogenous DNA-dependent RNA polymerase activity varied in the same manner, suggesting that RNA synthesis directed by enzyme II is modulated by chromosomal proteins.
在从未感染和感染弗瑞德病毒（FV）的脾细胞中分离出的细胞核中，在不同盐条件下观察到的酶II指导的RNA合成差异，被归因于染色体修饰（巴布科克和里奇，1973年）。进行这项研究是为了确定FV感染的脾脏染色质中是否发生了组成变化，这些变化与酶II合成速率的改变相关。对感染后不同时间染色质成分的定量研究表明，它们与分离细胞核中RNA合成速率以相同的时间方式变化。相对于DNA，RNA、组蛋白和非组蛋白在感染后14天达到最大值。随后在感染的剩余时间内逐渐下降。染色质内源性DNA依赖性RNA聚合酶活性以相同方式变化，表明酶II指导的RNA合成受染色体蛋白调节。
