Montagna R A, Wang T Y
Cancer Res. 1976 Sep;36(9 pt.1):3138-42.
The nonhistone proteins of Ehrlich ascites tumor chromatin have been separated into a loosely bound and two tightly bound protein fractions by sequential extraction of chromatin with 0.35 M NaCl and 2 M NaCl:5 M urea. The nonhistone proteins thus obtained were examined for their chemical composition and distribution of DNA polymerase, RNA polymerase, and protein kinase activities. In addition, the effect of these nonhistone proteins on transcription of DNA in vitro has been determined. The results indicate that these nonhistone proteins, fractionated on the basis of their extractability, exhibit varied compositional characteristics and play different functional roles in the synthesis of DNA and RNA and in the possible control of gene activity.
通过用0.35M氯化钠和2M氯化钠：5M尿素对艾氏腹水瘤染色质进行顺序提取，已将其非组蛋白分离为一个松散结合的蛋白组分和两个紧密结合的蛋白组分。对由此获得的非组蛋白进行了化学组成分析，并检测了DNA聚合酶、RNA聚合酶和蛋白激酶活性的分布。此外，还测定了这些非组蛋白对体外DNA转录的影响。结果表明，这些根据可提取性分级分离的非组蛋白具有不同的组成特征，并且在DNA和RNA合成以及可能的基因活性控制中发挥不同的功能作用。
