Peitras R J, Szego C M
Cancer Lett. 1976 Mar;1(4):237-41. doi: 10.1016/s0304-3835(75)97238-9.
Membrane alterations were detected in isolated epithelial cells treated in vitro with 5 x 10(-4) M dibutylnitrosamine (DBN) or 1.2 x 10(-9) M diethylstilbestrol (DES) for 30 min. Addition of DBN to bladder cells and DES to endometrial cells elicited a striking increment in Con A-mediated hemadsorption, but not Con A binding, to the treated cells as compared to those not treated with carcinogens. Concomitantly, release of the lysosomal proteinase, cathepsin B1, to the extracellular medium was essentially doubled in the preparations exposed to the carcinogens, as compared to corresponding controls. The increased cellular hemagglutination in response to carcinogen treatment was significantly reduced by prior incubation of the epithelial cells with ovomucoid, a proteinase inhibitor that suppresses the activity of cathepsin B1.
在用5×10⁻⁴ M二丁基亚硝胺（DBN）或1.2×10⁻⁹ M己烯雌酚（DES）体外处理30分钟的分离上皮细胞中检测到膜改变。与未用致癌物处理的细胞相比，向膀胱细胞中添加DBN以及向子宫内膜细胞中添加DES会引起刀豆球蛋白A（Con A）介导的对处理细胞的血细胞吸附显著增加，但Con A结合没有增加。同时，与相应对照相比，暴露于致癌物的制剂中溶酶体蛋白酶组织蛋白酶B1释放到细胞外培养基中的量基本增加了一倍。在用卵类粘蛋白（一种抑制组织蛋白酶B1活性的蛋白酶抑制剂）预先孵育上皮细胞后，致癌物处理引起的细胞血凝增加显著降低。
