Kakizoe T, Komatsu H, Niijima T, Kawachi T, Sugimura T
Cancer Res. 1980 Jun;40(6):2006-9.
The agglutination by concanavalin A of isolated epithelial cells of the rat bladder was examined after in vivo treatment of rats with various bladder carcinogens for one week. The carcinogens tested were N-butyl-N-(4-hydroxybutyl)nitrosamine, dibutylnitrosamine, N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide, 2-acetylaminofluorene, 2-napthylamine, benzidine, N-methyl-N-nitrosourea, and cyclophosphamide, and they were given to male Wistar rats p.o., s.c., intravesically, or i.p. As negative controls, the effects of administration of 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide, dimethylnitrosamine, N-methyl-N'-nitro-N-nitrosoguanidine, and surgical implantation of glass beads in the bladder were also tested. One week after the start of treatment, epithelial cells were isolated from the bladder by sonication, and agglutination of the isolated cells with concanavalin A was assayed. The observed agglutinabilities of isolated cells were found to be closely correlated with the reported bladder carcinogenicities of these chemicals in rats. Thus, concanavalin A agglutination of bladder cells should be a useful rapid in vivo mammalian system for screening bladder carcinogens.
在用各种膀胱致癌物对大鼠进行一周的体内处理后，检测了伴刀豆球蛋白A对大鼠膀胱分离上皮细胞的凝集作用。所测试的致癌物有N-丁基-N-(4-羟基丁基)亚硝胺、二丁基亚硝胺、N-[4-(5-硝基-2-呋喃基)-2-噻唑基]甲酰胺、2-乙酰氨基芴、2-萘胺、联苯胺、N-甲基-N-亚硝基脲和环磷酰胺，将它们经口、皮下、膀胱内或腹腔内给予雄性Wistar大鼠。作为阴性对照，还测试了给予2-(2-呋喃基)-3-(5-硝基-2-呋喃基)丙烯酰胺、二甲基亚硝胺、N-甲基-N'-硝基-N-亚硝基胍以及在膀胱内手术植入玻璃珠的效果。处理开始一周后，通过超声处理从膀胱分离上皮细胞，并测定分离细胞与伴刀豆球蛋白A的凝集作用。发现分离细胞的观察到的凝集性与这些化学物质在大鼠中已报道的膀胱致癌性密切相关。因此，膀胱细胞的伴刀豆球蛋白A凝集作用应该是一种用于筛选膀胱致癌物的有用的快速体内哺乳动物系统。
