完整动物体内的DNA损伤与修复。给予二甲基亚硝胺后大鼠肝脏DNA中的单链区域。

DNA damage and repair in intact animals. Single-stranded regions in rat liver DNA following administration of dimethylnitrosamine.

作者信息

Huang P H, Stewart B W

出版信息

Cancer Lett. 1976 May;1(5):263-8. doi: 10.1016/s0304-3835(75)97573-4.

Abstract

Rat liver DNA was radioactively labelled by administration of [3H]thymidine following partial hepatectomy. Two weeks later, the rats were treated with the carcinogen, dimethylnitrosamine. DNA was isolated and fractionated by elution from benzoylated DEAE-cellulose with NaCl and caffeine solution. The caffeine-eluted fraction was increased by administration of dimethylnitrosamine. This increase was proportional to the dose of carcinogen injected and persisted for at least 24 h after administration of the carcinogen. These data, together with the results of hydroxyapatite chromatography, suggest that the DNA contains short single-stranded sections associated with much longer regions of native DNA.

摘要

在部分肝切除术后，通过给予[3H]胸腺嘧啶核苷对大鼠肝脏DNA进行放射性标记。两周后，用致癌物二甲基亚硝胺处理这些大鼠。通过用氯化钠和咖啡因溶液从苯甲酰化的二乙氨基乙基纤维素上洗脱来分离和分级分离DNA。给予二甲基亚硝胺后，咖啡因洗脱级分增加。这种增加与注射的致癌物剂量成比例，并且在给予致癌物后至少持续24小时。这些数据与羟基磷灰石色谱分析结果一起表明，DNA含有与更长的天然DNA区域相关的短单链片段。