Kosic J, Stewart B W
Cancer Lett. 1983 Aug;20(1):5-12. doi: 10.1016/0304-3835(83)90180-5.
Structural analysis by chromatography on benzoylated-DEAE-cellulose (BD-cellulose) has been made of hepatic DNA from rats treated for up to 21 weeks with dimethylnitrosamine (DMN). The carcinogen (1 mg/kg body wt) was administered on a daily basis by intraperitoneal injection. By comparison with preparations from saline-treated controls, the proportion of DNA retained by benzoylated-DEAE-cellulose in the presence of 1.0 M NaCl was increased by administration of the carcinogen. Variation in the result, dependent upon the time after the final dose, suggested a complex relationship between structural damage to DNA and duration of treatment. Structural damage was confirmed by S1 nuclease digestion. The observations imply that in the course of chronic administration, increasing time is required to complete DNA repair processes operative in rat liver.
对用二甲基亚硝胺（DMN）处理长达21周的大鼠肝脏DNA进行了苯甲酰化-二乙氨基乙基纤维素（BD-纤维素）柱层析结构分析。致癌物（1毫克/千克体重）通过腹腔注射每日给药。与生理盐水处理的对照组制备物相比，在1.0M氯化钠存在下，苯甲酰化-二乙氨基乙基纤维素保留的DNA比例因致癌物给药而增加。结果的变化取决于最后一剂后的时间，这表明DNA结构损伤与治疗持续时间之间存在复杂关系。S1核酸酶消化证实了结构损伤。这些观察结果表明，在慢性给药过程中，大鼠肝脏中进行DNA修复过程所需的时间越来越长。
