Arima T, Motoyama Y, Nagata K, Kondo T
Gastroenterol Jpn. 1976;11(4):300-6.
An improved method for quantitation of desialylated glycoproteins was developed. The assay was carried out in non-saturating condition other than saturating condition which has been used by the other authors. In the present method a lower radioactivity in the assay solution can be used. Standard curves obtained by adding an increasing amount of desialylated alpha1-acid glycoprotein to the assay solution made it possible to measure desialylated glycoproteins equivalent to 5-200 ng of desialylated alpha1-acid glycoprotein. Desialylated alpha1-acid glycoprotein used for the assay could be prepared in large scale by hydrolysis with 0.1 N-H2SO4.
开发了一种改进的去唾液酸糖蛋白定量方法。该测定是在非饱和条件下进行的，而不是其他作者所使用的饱和条件。在本方法中，可以使用测定溶液中较低的放射性。通过向测定溶液中添加越来越多的去唾液酸α1-酸性糖蛋白获得的标准曲线使得能够测量相当于5-200 ng去唾液酸α1-酸性糖蛋白的去唾液酸糖蛋白。用于该测定的去唾液酸α1-酸性糖蛋白可以通过用0.1 N - H2SO4水解大规模制备。
