Young W V, Heemsoth C H, Georgiafandis G, Mitchell D C, Hackett D K, Bahna D G
Baptist Hospital of East Tennessee, Department of Perfusion, Knoxville 37920, USA.
J Extra Corpor Technol. 1997 Dec;29(4):181-4.
One of the most important tasks of the perfusionist is the proper assembly of the extracorporeal circuit (ECC) prior to the initiation of cardiopulmonary bypass (CPB). The ECC is usually assembled, primed and debubbled 30 minutes to one hour prior to the patient entering the operating room. But there are occasions when the ECC may have been set up and the previously scheduled procedure cancelled. Perfusionists in this situation have found themselves in a quandary; dispose of the ECC because of required nursing compliance and the sterility question, or keep it and use it later because of the economic impact on the "bottom line". Some hospitals may have satisfactorily answered the question of ECC sterility after 24 hours without observation, but the few reported papers regarding this issue, and our desire to save these circuits, inspired us to find out if they were in fact sterile after having been open for a long period of time. The purpose of this study was to evaluate ECC sterility using an open reservoir oxygenator, over a time period of seven days. After obtaining 792 bacterial cultures from three sites within the ECC, the study was terminated. There were no positive bacterial cultures during the study period. Assuming there is no deliberate contamination, pump circuits assembled in an unused operating room can be maintained sterile for a period of seven days.
体外循环灌注师最重要的任务之一是在启动体外循环（CPB）之前正确组装体外循环回路（ECC）。ECC通常在患者进入手术室前30分钟至1小时组装、预充并除泡。但有时会出现ECC已搭建好而先前安排的手术取消的情况。处于这种情况的灌注师会陷入两难境地：由于护理要求和无菌问题而丢弃ECC，还是由于对“底线”的经济影响而保留并稍后使用。一些医院可能在未观察24小时后就令人满意地回答了ECC无菌性的问题，但关于这个问题的报道论文很少，而且我们希望节省这些回路，这促使我们去弄清楚它们在长时间开放后是否真的无菌。本研究的目的是使用开放储液器氧合器在七天的时间段内评估ECC的无菌性。在从ECC内的三个部位获取792份细菌培养样本后，该研究终止。研究期间没有阳性细菌培养结果。假设没有故意污染，在未使用的手术室中组装的泵回路可保持无菌七天。
