Lonský V, Voxová B, Dominik J, Mandák J, Kubícek J, Bímová J, Marková D, Matoulková P
Department of Cardiac Surgery, Charles University, Faculty of Medicine and Teaching Hospital, Hradec Králové.
Acta Medica (Hradec Kralove). 1998;41(2):91-3.
The sterility of previously assembled cardiopulmonary bypass circuits was investigated for 100 extracorporeal circuits. The closed circuits were assembled using aseptic technique and remained in the pump room until time of use. The mean time from point of setup to point of priming for the 100 consecutive circuits was 32 hours, with a range of 19 to 89 hours. Circuits were primed with the calculated volume of priming solution, circulated for 5 minutes and tested for microbial contamination by withdrawing 20 ml of the priming solution and 10 days incubated in Thioglycolate and Sabouraud culture mediums. All were found to be free of microbial contamination. The results of this investigation demonstrate that the sterility of the extracorporeal circuit, pre-assembled in advance of actual priming, can be maintained over an extended interval when standard aseptic technique is used. This allows the utilization of a pre-assembled circuit for emergency cardiopulmonary support.
对100个体外循环回路的先前组装的心肺旁路回路的无菌性进行了研究。封闭回路采用无菌技术组装，并一直存放在泵室中直至使用。这100个连续回路从设置点到预充点的平均时间为32小时，范围为19至89小时。用计算好体积的预充液对回路进行预充，循环5分钟，通过抽取20毫升预充液并在硫乙醇酸盐和沙氏培养基中培养10天来检测微生物污染情况。结果发现所有回路均无微生物污染。该研究结果表明，在实际预充前预先组装的体外回路，当使用标准无菌技术时，其无菌性可在较长时间内保持。这使得预组装回路可用于紧急心肺支持。
