Rechtoris C, McIntosh A H
In Vitro. 1976 Oct;12(10):678-81. doi: 10.1007/BF02797470.
Spent media from five different insect cell lines when inoculated into Trichoplusia ni (TN-368) cultures produced cytotoxicity resulting in rounding and detachment of cells. The substance in spent medium from the established cellline Carpocapsa pomonella (CP-169) is believed to be a toxin, based on the failure to serially passage the agent, the early appearance of the cytotoxic effect, and the inability to detect microbes by culturing techniques as well as by electron microscopy. The ability to extract the toxic substance from CP-169 cells indicates that it is cell associated. Biophysical and biochemical properties of the CP-169 cytotoxin are presented.
将来自五种不同昆虫细胞系的用过的培养基接种到粉纹夜蛾（TN - 368）培养物中时，会产生细胞毒性，导致细胞变圆并脱落。基于该病原体无法连续传代、细胞毒性效应出现较早以及通过培养技术和电子显微镜均无法检测到微生物，已建立的细胞系苹果蠹蛾（CP - 169）的用过的培养基中的物质被认为是一种毒素。从CP - 169细胞中提取有毒物质的能力表明它与细胞相关。本文介绍了CP - 169细胞毒素的生物物理和生化特性。
