Minassian I, Bell L G
J Cell Sci. 1976 Dec;22(3):521-30. doi: 10.1242/jcs.22.3.521.
Light- and electron-microscope autoradiography have been used to follow the incorporation of [3H]thymidine at different stages during the interphase of synchronously growing populations of Amoeba proteus. Two main patterns were found for tritiated thymidine incorporation, i.e. DNA synthesis. The major incorporation was in the central region of the nucleus, but a lesser degree of incorporation occurred in the nucleolar region. The bulk of this nucleolar DNA was found to be late replicating, i.e. it replicated during the G2 phase.
利用光学显微镜和电子显微镜放射自显影技术，追踪了同步生长的变形虫种群在间期不同阶段[3H]胸腺嘧啶核苷的掺入情况。发现了氚标记胸腺嘧啶核苷掺入的两种主要模式，即DNA合成。主要掺入发生在细胞核的中央区域，但核仁区域的掺入程度较低。发现大部分核仁DNA复制较晚，即在G2期复制。
