Patterson S, Goldspink G
J Cell Sci. 1976 Dec;22(3):607-16. doi: 10.1242/jcs.22.3.607.
The mechanisms of myofibril growth proliferation were investigated in the red and white muscles of fish. In both types of muscle the ratio of lattice filament spacings between the Z disk and M line was found to be greater than that required for perfect transformation of a square into a hexagonal lattice. This mismatch was considered to result in the thin filaments being pulled obliquely instead of at right angles to the Z disk. The angle of pull of the thin filaments was measured in longitudinal sections. The splitting process was found to decrease the degree of pull. Splitting was also observed in transverse sections of the peripheral myofibrils. In both red and white fibres these myofibrils were found to commence splitting when they reached a size of approximately 1-2 mum diameter. Evidence from ultrastructural and autoradiographical studies suggested that growth of the myofibrils within the fibres is centrifugal. The outermost myofibrils appear to be the ones which are being built up and which split. The data indicated that in fish muscle a considerable number of filaments may be added to the daughter regions whilst splitting of the myofibril is still continuing.
对鱼类红肌和白肌中肌原纤维生长增殖的机制进行了研究。在这两种类型的肌肉中，发现Z盘和M线之间的晶格细丝间距比将正方形完美转变为六边形晶格所需的间距大。这种不匹配被认为会导致细肌丝倾斜拉动，而不是与Z盘成直角拉动。在纵切面中测量了细肌丝的拉动角度。发现分裂过程会降低拉动程度。在周边肌原纤维的横切面中也观察到了分裂现象。在红色和白色纤维中，这些肌原纤维在直径达到约1-2微米大小时开始分裂。超微结构和放射自显影研究的证据表明，纤维内肌原纤维的生长是离心式的。最外层的肌原纤维似乎是正在形成并发生分裂的肌原纤维。数据表明，在鱼类肌肉中，在肌原纤维仍在持续分裂时，可能会有相当数量的细丝添加到子区域。
