Ghadersohi A, Hirst R G, Forbes-Faulkener J, Coelen R J
Department of Microbiology and Immunology, Australian Institute of Tropical Veterinary and Animal Sciences, James Cook University, Townsville.
Vet Microbiol. 1999 Mar 12;65(3):185-94. doi: 10.1016/s0378-1135(98)00297-1.
A highly sensitive and specific PCR (MB-PCR) was used in preliminary studies to detect M. bovis in milk samples to investigate its association with high somatic cell count (SCC), an indicator of subclinical mastitis and one of the factors in down grading the quality of milk. A total of 186 and 167 herds were tested with 43% and 62% of herds positive for M. bovis in Victoria and North Queensland, respectively. The quarter milks from 52 cows with persistently high SCC were tested by MB-PCR and culture to investigate the association of M. bovis with major mastitis pathogens (MMP). M. Bovis was detected in 77% of cows of which 19% alone had M. bovis without any other bacteria, 17% had M. bovis in combination with major mastitis pathogens and 40% had M. bovis in combination with non-major mastitis pathogens. We believe that M. bovis is widespread in dairy cattle and has the potential to produce disease alone or to predispose the udder to disease caused by major mastitis and environmental pathogens. These studies have revealed a hitherto unrecognised high prevalence of M. bovis in dairy cattle in North Queensland and Victoria in Australia. These initial studies also give a clear association between M. bovis and elevated somatic cell counts.
在初步研究中，使用了一种高度灵敏且特异的聚合酶链式反应（MB-PCR）来检测牛奶样本中的牛分枝杆菌，以调查其与高体细胞计数（SCC）的关联。高体细胞计数是亚临床乳腺炎的一个指标，也是降低牛奶质量的因素之一。在维多利亚州和北昆士兰州，分别对186个和167个牛群进行了检测，牛分枝杆菌检测呈阳性的牛群分别占43%和62%。通过MB-PCR和培养对52头体细胞计数持续偏高的奶牛的四分乳进行检测，以调查牛分枝杆菌与主要乳腺炎病原体（MMP）之间的关联。在77%的奶牛中检测到了牛分枝杆菌，其中19%的奶牛仅感染了牛分枝杆菌，未感染任何其他细菌；17%的奶牛感染了牛分枝杆菌并伴有主要乳腺炎病原体；40%的奶牛感染了牛分枝杆菌并伴有非主要乳腺炎病原体。我们认为，牛分枝杆菌在奶牛中广泛存在，有可能单独引发疾病，或使乳房易患由主要乳腺炎病原体和环境病原体引起的疾病。这些研究揭示了澳大利亚北昆士兰州和维多利亚州奶牛中牛分枝杆菌的高流行率，而这一情况此前未被认识到。这些初步研究还明确了牛分枝杆菌与体细胞计数升高之间的关联。
