采用种特异性巢式PCR和培养法对新西兰散装罐牛奶进行牛支原体检测。

Survey of bulk tank milk in New Zealand for Mycoplasma bovis , using species-specific nested PCR and culture.

作者信息

McDonald W L, Rawdon T G, Fitzmaurice J, Bolotovski I, Voges H, Humphrey S, Fernando K, Canagasebey Y, Thornton R N, McIntyre L

机构信息

Investigation and Diagnostic Centre, Ministry of Agriculture and Forestry Biosecurity New Zealand, PO Box 40742, Upper Hutt 5140, New Zealand.

出版信息

N Z Vet J. 2009 Feb;57(1):44-9. doi: 10.1080/00480169.2009.36867.

DOI:10.1080/00480169.2009.36867

PMID:19252542

Abstract

AIM

To survey the dairy cattle population in New Zealand for the presence or absence of Mycoplasma bovis.

METHODS

A random cross-sectional survey of bulk tank milk from dairy herds in New Zealand based on regionally proportioned sampling, weighted towards herds with a high bulk tank milk somatic cell count (SCC) was used to detect M. bovis at a between-herd prevalence of 2%, with 99% confidence. Bulk tank milk samples collected on-farm were tested using a nested M. bovis PCR, and bacteriological culture employing enrichment in mycoplasma broth and direct plating onto mycoplasma agar.

RESULTS

Mycoplasma bovis was not detected in any of the 244 bulk tank milk samples by either PCR or culture.

CONCLUSIONS

This survey provides further evidence that M. bovis is not present in the dairy cattle population in New Zealand.

摘要

目的

调查新西兰奶牛群体中牛支原体的有无情况。

方法

基于区域比例抽样对新西兰奶牛场的散装罐牛奶进行随机横断面调查，抽样偏向散装罐牛奶体细胞计数（SCC）高的牛群，以检测牛支原体，群体间患病率为2%，置信度为99%。使用巢式牛支原体聚合酶链反应（PCR）对农场采集的散装罐牛奶样本进行检测，并采用支原体肉汤增菌和直接接种到支原体琼脂上的方法进行细菌培养。