Kazim K, Wild G, Ward A
Department of Immunology, University of Sheffield, Northern General Hospital, UK.
J Clin Lab Immunol. 1998;50(1):17-25.
Cytokines are a group of low molecular weight glycoproteins important in cellular signaling for various responses such as activation and proliferation. They separate the cellular responses into Th1 and Th2 pathways, where each pathway releases its own cytokine group. Interleukins are an important section of the cytokine group and consist of a number of members with their respective receptors such as interleukin-2 and interleukin-2 receptor (IL-2 and IL-2R, respectively). In this study, a soluble interleukin-2 receptor alpha (sIL-2R alpha) sandwich ELISA was developed and tested with different samples. This method was compared with other mono- and polyclonal antibodies that measure sIL-2R alpha molecule using the same subjects and recombinant sIL-2R alpha. The results showed that different antibody combinations measure different epitopes on the receptor molecule.
细胞因子是一类低分子量糖蛋白，在细胞信号传导中起着重要作用，参与各种反应，如激活和增殖。它们将细胞反应分为Th1和Th2途径，每条途径释放其自身的细胞因子组。白细胞介素是细胞因子组的一个重要部分，由许多成员及其各自的受体组成，如白细胞介素-2和白细胞介素-2受体（分别为IL-2和IL-2R）。在本研究中，开发了一种可溶性白细胞介素-2受体α（sIL-2Rα）夹心ELISA，并对不同样品进行了测试。该方法与使用相同受试者和重组sIL-2Rα测量sIL-2Rα分子的其他单克隆和多克隆抗体进行了比较。结果表明，不同的抗体组合可检测受体分子上不同的表位。
