血清白细胞介素-18、白细胞介素-10、肿瘤坏死因子-α及可溶性白细胞介素-2受体在慢性丙型肝炎患者中的作用

The roles of serum IL-18, IL-10, TNF-alpha and sIL-2R in patients with chronic hepatitis C.

作者信息

Jia Hong-Yu, Du Jie, Zhu Si-He, Ma Ying-Ji, Chen Huan-Yong, Yang Bao-Shan, Cai Hua-Feng

机构信息

Department of Infectious Diseases, First Clinical College, Harbin Medical University, Harbin 150001, China.

出版信息

Hepatobiliary Pancreat Dis Int. 2002 Aug;1(3):378-82.

PMID:14607710

Abstract

OBJECTIVE

To identify the roles of serum IL-18, IL-10, TNF-alpha and sIL-2R in the pathogenesis of chronic hepatitis C and the effects of interferon on the mentioned serum cytokines.

METHODS

The levels of IL-18, IL-10, TNF-alpha and sIL-2R were detected in 10 healthy controls, 24 asymptomatic HCV carriers, and 27 patients with chronic hepatitis C (before and after IFN treatment) by enzyme linked immunosorbent assay (ELISA).

RESULTS

The levels of IL-18, IL-10, TNF-alpha and sIL-2R in the patients of chronic hepatitis C were higher than those in the healthy controls (P<0.05) and in asymptomatic HCV carriers (P<0.05). The values of the mentioned cytokines showed a significant positive correlation to GPT. The levels of the mentioned cytokines decreased obviously after IFN treatment (P<0.05), while the serum levels of IL-10 and sIL-2R reduced in sequence in no-response group, partial-response group and complete-response group.

CONCLUSIONS

IL-18, IL-10, TNF-alpha and sIL-2R co-participate in the pathogenesis of chronic hepatitis C, and are used to evaluate the effect of IFN on the immune state of organisms, and IL-10 and sIL-2R are important for predicting the anti-viral efficacy of IFN.

摘要

目的

探讨血清白细胞介素-18（IL-18）、白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）及可溶性白细胞介素-2受体（sIL-2R）在慢性丙型肝炎发病机制中的作用以及干扰素对上述血清细胞因子的影响。

方法

采用酶联免疫吸附试验（ELISA）检测10名健康对照者、24例无症状丙型肝炎病毒（HCV）携带者及27例慢性丙型肝炎患者（干扰素治疗前后）血清中IL-18、IL-10、TNF-α及sIL-2R水平。

结果

慢性丙型肝炎患者血清中IL-18、IL-10、TNF-α及sIL-2R水平高于健康对照者（P<0.05）及无症状HCV携带者（P<0.05）。上述细胞因子水平与谷丙转氨酶（GPT）呈显著正相关。干扰素治疗后上述细胞因子水平明显下降（P<0.05），而无反应组、部分反应组及完全反应组血清中IL-10及sIL-2R水平依次降低。