Aydin S, Demir T, Oztürk Y, Başer K H
Department of Pharmacology, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey.
Phytother Res. 1999 Feb;13(1):20-3. doi: 10.1002/(SICI)1099-1573(199902)13:1<20::AID-PTR380>3.0.CO;2-J.
A screening study was performed on/by essential oils of Nepeta viscida Boiss and Nepeta italica L. using tail-flick and tail immersion (52.5 degrees C) methods. N. italica samples were collected from three different localities of Turkey. Surprisingly, only one of the essential oils showed significant activity, which was blocked by naloxone, indicating the involvement of opioid receptors. This was seen only with the mechanical but not the thermal algesic stimulus, suggesting a specific activity on opioid receptors, excluding mu receptors. The same, active essential oil also exhibited a non-competitive inhibition of acetylcholine contractions of isolated rat ileum but it was inactive on the isolated rat aorta. Furthermore, a correlation between the analgesic activity and the amount of 1,8-cineole was noticed.
使用甩尾法和尾浸法（52.5摄氏度）对粘毛荆芥和意大利荆芥的精油进行了筛选研究。意大利荆芥样本采集自土耳其的三个不同地区。令人惊讶的是，只有一种精油显示出显著活性，且该活性可被纳洛酮阻断，表明阿片受体参与其中。这仅在机械性而非热痛觉刺激中观察到，提示对阿片受体具有特异性活性，不包括μ受体。同样的这种活性精油还对离体大鼠回肠的乙酰胆碱收缩表现出非竞争性抑制作用，但对离体大鼠主动脉无活性。此外，还注意到镇痛活性与1,8-桉叶素含量之间存在相关性。
