Stern Y, Segal K, Medalia O, Feinmesser R
Department of Otolaryngology, Head and Neck Surgery, Rabin Medical Center, Petah Tiqva, Israel.
Int J Pediatr Otorhinolaryngol. 1998 Nov 15;46(1-2):67-70. doi: 10.1016/s0165-5876(98)00122-0.
To evaluate DNA ploidy in papillary thyroid carcinoma in children in correlation to the clinical course of the disease.
Flow cytometric DNA ploidy measurements were performed on formalin-fixed, paraffin-embedded tumor specimens from 14 children and 14 adult patients with papillary carcinoma of the thyroid gland. Analysis of DNA content was performed blind to patient's age and clinical presentation.
Seven patients presented with cervical metastasis, one patient had distal metastasis and four patients had local invasion. All patients underwent total thyroidectomy. Seven children underwent bilateral modified neck dissection. Twenty-five tumors expressed diploid DNA content. No statistically significant difference in DNA content was observed between the tumors from child and adult patients. No correlation was found between DNA content and aggressive presentation in the pediatric group.
Our primary results indicate that diploid DNA content is common in papillary thyroid carcinoma in children and aggressive clinical presentation is not associated with DNA aneuploidy. Larger prospective studies and long-term clinical follow-up is warranted to document the clinical significance of these observations.
评估儿童甲状腺乳头状癌的DNA倍体情况及其与疾病临床进程的相关性。
对14例儿童和14例成人甲状腺乳头状癌患者经福尔马林固定、石蜡包埋的肿瘤标本进行流式细胞术DNA倍体检测。DNA含量分析在对患者年龄和临床表现不知情的情况下进行。
7例患者出现颈部转移，1例患者有远处转移，4例患者有局部侵犯。所有患者均接受了甲状腺全切术。7例儿童接受了双侧改良颈清扫术。25个肿瘤表现为二倍体DNA含量。儿童和成人患者肿瘤的DNA含量无统计学显著差异。在儿童组中，未发现DNA含量与侵袭性表现之间存在相关性。
我们的初步结果表明，二倍体DNA含量在儿童甲状腺乳头状癌中很常见，侵袭性临床表现与DNA非整倍体无关。有必要进行更大规模的前瞻性研究和长期临床随访，以记录这些观察结果的临床意义。
