Ament W, Huizenga J R, Kort E, van der Mark T W, Grevink R G, Verkerke G J
Division of Artificial Organs, Biomedical Technology, Faculty of Medical Sciences, University of Groningen, The Netherlands.
Int J Sports Med. 1999 Feb;20(2):71-7. doi: 10.1055/s-2007-971096.
The aim of this study was to investigate whether the increase of ammonia concentration and lactate concentration in blood was accompanied by an increased expiration of ammonia during graded exercise. Eleven healthy subjects performed an incremental cycle ergometer test. Blood ammonia, blood lactate and the amount of expired ammonia were measured until 30 minutes post exercise. The expired air was guided through a flow chamber filled with a sulphuric acid solution to trap the expired ammonia. Blood ammonia, blood lactate increased more than proportionally and the amount of expired ammonia (in micromol/min) increased exponentially with the workload. Post-exercise the amount of expired ammonia decreased within a few minutes back to pre-exercise levels while the concentrations of lactate and ammonia in blood decreased much more slowly and were still elevated after 30 minutes of recovery. We conclude that the more than proportional increase of ammonia and lactate during graded exercise, is accompanied with an exponential increase of expired ammonia output. Faster and more accurate ammonia gas detection techniques are necessary to quantify more precisely the respiratory ammonia output during graded exercise.
本研究的目的是调查在分级运动过程中，血液中氨浓度和乳酸浓度的升高是否伴随着呼出氨量的增加。11名健康受试者进行了递增式蹬车测试。在运动后30分钟内测量血氨、血乳酸和呼出氨量。呼出的气体通过一个装有硫酸溶液的流动腔，以捕获呼出的氨。血氨、血乳酸的增加超过了比例，呼出氨量（以微摩尔/分钟计）随工作量呈指数增加。运动后，呼出氨量在几分钟内降至运动前水平，而血液中乳酸和氨的浓度下降得慢得多，恢复30分钟后仍处于升高状态。我们得出结论，分级运动过程中氨和乳酸超过比例的增加伴随着呼出氨量的指数增加。需要更快、更准确的氨气检测技术，以更精确地量化分级运动过程中的呼吸氨输出量。
