Mitchell R L, Buckland R B
Poult Sci. 1976 Nov;55(6):2195-200. doi: 10.3382/ps.0552195.
The use of dimethyl sulfoxide (DMSO) as a cryoprotective agent for freezing chicken semen was examined with respect to intravaginal (I.V.) and intrauterine(I.U.) inseminations. Semen diluted in DMSO was subjected to various freeze rates, equilibration times and thawing rates. Various concentrations of DMSO were examined as was the effect of diluting DMSO with phosphate buffer and Lake's solution. There was no significant (p greater than 0.05) effect of rate of freeze (ranging from 1.3 degrees C to 7.2 degrees C. per min) on fertility. However, rapid thawing in an ice bath resulted in a longer (p less than 0.05) duration of fertility than thawing at 1 degree C. per min., but had no effect on percent fertility during duration or percent hens fertile. Equilibration time and concentration of DMSO had no significant (p greater than 0.05) effects on fertility. There was no interaction between insemination route and Lake's solution vs. phosphate buffer with respect to duration of fertility. It was concluded that intrauterine (I.U.) insemination resulted in superior fertility to intravaginal (I.V.) inseminations with semen frozen in DMSO.
就阴道内（I.V.）和子宫内（I.U.）授精而言，研究了使用二甲基亚砜（DMSO）作为冷冻鸡精液的冷冻保护剂的情况。用DMSO稀释的精液经受了各种冷冻速率、平衡时间和解冻速率。研究了不同浓度的DMSO以及用磷酸盐缓冲液和莱克氏溶液稀释DMSO的效果。冷冻速率（每分钟1.3摄氏度至7.2摄氏度）对受精率没有显著（p大于0.05）影响。然而，在冰浴中快速解冻导致受精持续时间比每分钟1摄氏度解冻更长（p小于0.05），但对持续期间的受精率百分比或产蛋母鸡受精百分比没有影响。平衡时间和DMSO浓度对受精率没有显著（p大于0.05）影响。就受精持续时间而言，授精途径与莱克氏溶液和磷酸盐缓冲液之间没有相互作用。得出的结论是，对于用DMSO冷冻的精液，子宫内（I.U.）授精的受精率优于阴道内（I.V.）授精。
