Latorre J R, Harris G C, Johnson Z B
Department of Animal Sciences, University of Arkansas, Fayetteville 72701.
Poult Sci. 1988 Feb;67(2):333-5. doi: 10.3382/ps.0670333.
Pooled semen was diluted with Lake's solution containing 10.8% (v/v) glycerol. Aliquots of 1.2 mL and .5 mL were transferred to 1.8-mL capacity plastic vials and .5-mL capacity plastic straws, respectively. Semen was processed at 5 C, frozen by cooling at a rate of 3 C/min to -35 C, then plunged in liquid nitrogen. Samples were thawed at 5 C, diluted stepwise, centrifuged, and resuspended with nonglycerolized diluent at 5 C. Fertility and its duration were measured after single or double (insemination on 2 consecutive days) insemination. No significant differences were found in fertility percentages between semen samples frozen and stored in the two types of containers. However, fertility percentages were significantly higher for eggs laid on Days 2 to 5 after double insemination than for eggs laid after single insemination. No such differences were noted in the fertility of eggs laid on Days 2 to 7. Duration of fertility was significantly longer for semen frozen in plastic vials than for semen frozen in straws.
将混合精液用含有10.8%（体积/体积）甘油的莱克氏溶液稀释。分别将1.2毫升和0.5毫升的等分试样转移至容量为1.8毫升的塑料小瓶和容量为0.5毫升的塑料细管中。精液在5℃下处理，以3℃/分钟的速率冷却至-35℃进行冷冻，然后投入液氮中。样品在5℃下解冻，逐步稀释，离心，并在5℃下用无甘油稀释剂重悬。在单次或两次（连续两天输精）输精后测量受精率及其持续时间。冷冻并储存在两种类型容器中的精液样本之间的受精率没有显著差异。然而，两次输精后第2至5天产下的卵的受精率明显高于单次输精后产下的卵。在第2至7天产下的卵的受精率方面没有观察到此类差异。塑料小瓶中冷冻的精液的受精持续时间明显长于细管中冷冻的精液。
