Kingsley D H, Keeler C L
Department of Animal and Food Sciences, University of Delaware, Newark, Delaware, 19717-1303, USA.
Virology. 1999 Apr 10;256(2):213-9. doi: 10.1006/viro.1999.9609.
Among the alpha herpesviruses studied to date, the initial stage of wild-type virus attachment involves an interaction between virally encoded structural envelope glycoproteins (predominantly glycoprotein C) and cell surface heparan sulfate proteoglycans. An analysis of the infectious laryngotracheitis virus (ILTV) glycoprotein C and glycoprotein B sequences suggested that these proteins lacked consensus heparin-binding domains. This indicated that ILTV might attach to its host cell in a heparan-independent manner, distinct from other alpha herpesviruses. The infectivity of two ILTV strains, a tissue-culture-adapted vaccine strain and a virulent field challenge strain, were found to be insensitive to the presence of exogenous heparin or chondroitin. Furthermore, infectivity was retained in chicken embryonic liver cells treated with heparinase. However, 4 degrees C attachment studies and penetration studies in the presence of citrate buffer clearly demonstrated that ILTV attaches stably to and effectively penetrates chicken embryonic liver cells. Consequently, ILTV represents an alpha herpesvirus whose initial attachment step does not involve interactions with heparan or chondroitin sulfate containing proteoglycans.
在迄今为止研究的甲型疱疹病毒中，野生型病毒附着的初始阶段涉及病毒编码的结构性包膜糖蛋白（主要是糖蛋白C）与细胞表面硫酸乙酰肝素蛋白聚糖之间的相互作用。对传染性喉气管炎病毒（ILTV）糖蛋白C和糖蛋白B序列的分析表明，这些蛋白缺乏共有肝素结合结构域。这表明ILTV可能以一种不依赖硫酸乙酰肝素的方式附着于其宿主细胞，这与其他甲型疱疹病毒不同。发现两种ILTV毒株，一种适应组织培养的疫苗毒株和一种强毒力的田间攻击毒株，其感染性对外源肝素或硫酸软骨素的存在不敏感。此外，在用肝素酶处理的鸡胚肝细胞中仍保留感染性。然而，4℃附着研究和在柠檬酸盐缓冲液存在下的穿透研究清楚地表明，ILTV能稳定附着并有效穿透鸡胚肝细胞。因此，ILTV是一种甲型疱疹病毒，其初始附着步骤不涉及与含硫酸乙酰肝素或硫酸软骨素的蛋白聚糖的相互作用。
