Chan F L, Wong Y C
Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories.
Histochem J. 1998 Jun;30(6):447-59. doi: 10.1023/a:1003264007923.
In the present study, the expression of glycoconjugates in the guinea pig seminal vesicle was localized and partially characterized by lectin histochemistry using a battery of 30 different lectins specific for different carbohydrate residues. The results indicate that the glandular epithelium of the guinea pig seminal vesicle exhibits complex glycoconjugates rich in Man, beta-GlcNAc, beta-Gal, alpha/beta-GalNAc, Fuc and complex NeuAc(alpha2,6)Gal/GalNAc residues, as shown by its positive reactions to most lectins used. The Golgi region of the luminal secretory epithelial cells expresses a complex glycoconjugate pattern, as shown by its strong reactions to Man-(PSA, GNA), beta-GlcNAc-(S-WGA, PWA, DSA, UDA), beta-Gal-(RCA-I and -II), alpha/beta-GalNAc-(SBA, Jac, VVA, BPA) and complex NeuAc-(SNA) specific lectins, indicating that the secretory epithelial cells are active in glycosylation and secretion process. It was also shown in the present study that the basal and luminal epithelial cells are different in their glycoconjugates. The basal epithelial cells are rich in NeuAc(alpha2,3)Gal residues as they are stained specifically by MAA. The fibroblasts in the epithelial-smooth muscle interface and the smooth muscle cells close to the glandular epithelium are shown to express more glycoconjugates as they are stained intensely by GS-I-B4, GS-II and SBA. However, their role in the epithelial-stromal interaction in the seminal vesicle remains to be elucidated. In summary, the present study reports for the first time on the lectin binding patterns of the guinea pig seminal vesicle, and the results show that the seminal vesicle epithelium elaborates and secretes glycoconjugates in a complex pattern. Some of the lectins might be useful as histochemical markers for the secretory activity and specific structural components in the guinea pig seminal vesicle.
在本研究中，使用一系列针对不同碳水化合物残基的30种不同凝集素，通过凝集素组织化学对豚鼠精囊中的糖缀合物表达进行了定位并部分表征。结果表明，豚鼠精囊的腺上皮表现出富含甘露糖（Man）、β- N -乙酰葡糖胺（β-GlcNAc）、β-半乳糖（β-Gal）、α/β- N -乙酰半乳糖胺（α/β-GalNAc）、岩藻糖（Fuc）和复合唾液酸（NeuAc(α2,6)Gal/GalNAc）残基的复杂糖缀合物，这通过其对大多数所用凝集素的阳性反应得以显示。腔面分泌上皮细胞的高尔基体区域表达复杂的糖缀合物模式，这通过其对Man-（PSA、GNA）、β-GlcNAc-（S-WGA、PWA、DSA、UDA）、β-Gal-（RCA-I和-II）、α/β-GalNAc-（SBA、Jac、VVA、BPA）和复合NeuAc-（SNA）特异性凝集素的强烈反应得以显示，表明分泌上皮细胞在糖基化和分泌过程中具有活性。本研究还表明，基底上皮细胞和腔面上皮细胞的糖缀合物不同。基底上皮细胞富含NeuAc(α2,3)Gal残基，因为它们被MAA特异性染色。上皮-平滑肌界面的成纤维细胞和靠近腺上皮的平滑肌细胞显示出表达更多的糖缀合物，因为它们被GS-I-B4、GS-II和SBA强烈染色。然而，它们在精囊上皮-基质相互作用中的作用仍有待阐明。总之，本研究首次报道了豚鼠精囊的凝集素结合模式，结果表明精囊上皮以复杂模式合成并分泌糖缀合物。一些凝集素可能作为豚鼠精囊分泌活性和特定结构成分的组织化学标记物有用。
