日本小型（柴犬）山羊精囊的糖缀合物组织化学

Glycoconjugate histochemistry of the seminal vesicles of the Japanese miniature (Shiba) goat.

作者信息

Sakairi A, Tsukise A, Habata I, Nara T, Meyer W

机构信息

Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.

出版信息

J Vet Med A Physiol Pathol Clin Med. 2004 Mar;51(2):39-45. doi: 10.1111/j.1439-0442.2004.00599.x.

DOI:10.1111/j.1439-0442.2004.00599.x

PMID:15153071

Abstract

The present study localizes and characterizes complex glycoconjugates in the seminal vesicles of the Japanese Shiba goat, using several carbohydrate histochemical procedures, including lectin techniques at light and electron microscopic levels. Glandular epithelial cells and luminal secretions were shown to contain neutral and acidic glycoconjugates with various saccharide residues, such as alpha-D-Man, alpha-D-Glc, alpha-L-Fuc, beta-D-Gal, GalNAc, GlcNAc, and NeuAc (sialic acid). The terminal oligosaccharide chains of sialoglycoconjugates present in the seminal vesicles were NeuAc alpha 2-3Gal beta 1-3GalNAc and NeuAc alpha 2-3Gal beta 1-4GlcNAc. In addition, in lysosomes of the glandular epithelial cells alpha-D-Man, alpha-D-Glc, GlcNAc and NeuAc (sialic acid) residues could be detected, the secretory vesicles contained alpha-L-Fuc, and the endoplasmic reticulum exhibited alpha-D-Man and alpha-D-Glc residues. The complex glycoconjugates with various sugar residues found in the seminal vesicles of the goat may be involved in various fertilization-related events.

摘要

本研究采用多种碳水化合物组织化学方法，包括光镜和电镜水平的凝集素技术，对日本柴山羊精囊中的复合糖缀合物进行定位和表征。结果显示，腺上皮细胞和管腔分泌物中含有带有各种糖残基的中性和酸性糖缀合物，如α-D-甘露糖、α-D-葡萄糖、α-L-岩藻糖、β-D-半乳糖、N-乙酰半乳糖胺、N-乙酰葡糖胺和N-乙酰神经氨酸（唾液酸）。精囊中存在的唾液酸糖缀合物的末端寡糖链为NeuAcα2-3Galβ1-3GalNAc和NeuAcα2-3Galβ1-4GlcNAc。此外，在腺上皮细胞的溶酶体中可检测到α-D-甘露糖、α-D-葡萄糖、N-乙酰葡糖胺和N-乙酰神经氨酸（唾液酸）残基，分泌小泡中含有α-L-岩藻糖，内质网中显示有α-D-甘露糖和α-D-葡萄糖残基。山羊精囊中发现的带有各种糖残基的复合糖缀合物可能参与各种与受精相关的事件。