Ceccarelli C, Santini D, Chieco P, Taffurelli M, Gamberini M, Pileri S A, Marrano D
Laboratorio di Immunocitopatologia Oncologica, Università di Bologna, Bologna, Italy.
J Clin Pathol. 1998 Nov;51(11):818-24. doi: 10.1136/jcp.51.11.818.
To investigate the expression of retinoblastoma protein (pRb) in invasive breast tumours and compare its expression with the major biopathological prognostic indicators to identify more aggressive subgroups.
Archival paraffin embedded tissues from 153 consecutive primary breast carcinomas.
pRb, Ki-67, and oestrogen receptor/progesterone receptor proteins were identified by immunohistochemistry and score values were recorded by image cytometric analysis; p53 and EGFr expression was also evaluated.
pRb scores correlated strongly with proliferation activity as determined by Ki-67 staining. Positive relations were also observed between pRb scores, tumour size, nuclear and histological grade, and oestrogen receptor/progesterone receptor content, while abnormal p53 accumulation was not associated with pRb expression. Among the high proliferating carcinomas it was possible to identify 13 cases with loss of pRb expression.
pRb expression paralleled proliferative activity in the majority of breast carcinomas examined, suggesting that in these cases the protein behaves normally in regulating the cell cycle. Conversely in cases with loss of pRb immunostaining, the combined expression of specific highly aggressive factors (EGFr and p53 expression, oestrogen receptor/progesterone receptor negative status, and high K67) seems to characterise a more aggressive phenotype showing growth advantage and cellular "progression" rather than significant nodal involvement.
研究视网膜母细胞瘤蛋白（pRb）在浸润性乳腺癌中的表达，并将其表达与主要生物病理学预后指标进行比较，以识别更具侵袭性的亚组。
来自153例连续原发性乳腺癌的存档石蜡包埋组织。
通过免疫组织化学鉴定pRb、Ki-67和雌激素受体/孕激素受体蛋白，并通过图像细胞分析记录评分值；同时评估p53和表皮生长因子受体（EGFr）的表达。
pRb评分与Ki-67染色所确定的增殖活性密切相关。在pRb评分、肿瘤大小、核分级和组织学分级以及雌激素受体/孕激素受体含量之间也观察到正相关，而p53异常积聚与pRb表达无关。在高增殖性癌中，有可能识别出13例pRb表达缺失的病例。
在所检查的大多数乳腺癌中，pRb表达与增殖活性平行，表明在这些病例中该蛋白在调节细胞周期方面表现正常。相反，在pRb免疫染色缺失的病例中，特定高侵袭性因子（EGFr和p53表达、雌激素受体/孕激素受体阴性状态以及高K67）的联合表达似乎表征了一种更具侵袭性的表型，显示出生长优势和细胞“进展”，而非显著的淋巴结受累。
